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Literature summary for 4.3.2.1 extracted from

  • Xia, J.; Yamaji, N.; Che, J.; Shen, R.F.; Ma, J.F.
    Normal root elongation requires arginine produced by argininosuccinate lyase in rice (2014), Plant J., 78, 215-226.
    View publication on PubMed
Search for more literature for 4.3.2.1

Cloned(Commentary)

Cloned (Comment) Organism
gene OsASL1, two variants OsASL1.1 and OsASL1.2 resulting from different transcription start sites, map-based cloning, only OsASL1.1 is able to complement the ref1 mutant phenotype, functional expression of OsASL1.1 in Saccharomyces cerevisiae, plastid-localized expression of OsASL1.1-GFP fusion protein in onion epidermal cells Oryza sativa

Protein Variants

Protein Variants Comment Organism
additional information isolation of the osred1, i.e. Oryza sativa root elongation defect, mutant from a short root-phenotype Tos-17 transposon insertion line, NE7046 Oryza sativa
R140L naturally occuring mutation of variant OsASL1.1, which renders the enzyme catalytically inactive Oryza sativa

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast expression of OsASL1.1 Oryza sativa 9507
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plastid expression of OsASL1.1 Oryza sativa 9536
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Organism

Organism UniProt Comment Textmining
Oryza sativa
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 cv. Nipponbare, gene OsASL1
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Source Tissue

Source Tissue Comment Organism Textmining
additional information expression patterns of OsASL1.1 and OsALS1.2, abundance of OsALS1.2 is much lower than that of OsASL1.1 in either tissue, overview Oryza sativa
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root expression of enzyme genetic variant OsASL1.1 Oryza sativa
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shoot expression of enzyme genetic variant OsASL1.1 Oryza sativa
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the genetic variant OsASL1.1 has argininosuccinate lyase activity, while variant OsASL1.2 has no activity Oryza sativa ?
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 ?

Synonyms

Synonyms Comment Organism
AL
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 Oryza sativa

General Information

General Information Comment Organism
malfunction a point mutation of a gene encoding argininosuccinate lyase causes a short root phenotype in the root elongation defect 1 mutant ref1, the enzyme activity is lost by the mutation R140L in OsASL1.1. The mutant has a shorter length from the quiescent center to the starting point of the elongation zone but a similar cell size and number of lateral and crown roots, and similar radial structure and nutrient uptake patterns compared to the wild-type. The mutant phenotype can be rescued by exogenous addition of arginine, but not by other amino acids Oryza sativa
metabolism the enzyme catalyzes the last step of arginine biosynthesis Oryza sativa
physiological function the enzyme is required for arginine production for use in normal root elongation in rice Oryza sativa