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Literature summary for 4.3.2.1 extracted from

  • Tsai, M.; Koo, J.; Howell, P.L.
    Recovery of argininosuccinate lyase activity in duck delta1 crystallin (2005), Biochemistry, 44, 9034-9044.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed as His tag enzymes Anas sp.

Protein Variants

Protein Variants Comment Organism
A41G/I43M mutant without enzyme activity Anas sp.
D115A mutant with 45% enzyme activity Anas sp.
D115E mutant with 42.6% enzyme activity Anas sp.
E115D mutant without enzyme activity Anas sp.
I143M mutant without enzyme activity Anas sp.
M9F/E115D mutant with 3.3% enzyme activity Anas sp.
M9W mutant with 2.0% enzyme activity Anas sp.
M9W/E115D mutant with 17% enzyme activity Anas sp.
M9W/V14S mutant with 1.9% enzyme activity Anas sp.
M9Y/E115D mutant with 5.5% enzyme activity Anas sp.
TRUN1-8 mutant with 37% enzyme activity Anas sp.
TRUN1-9 mutant with 2.1% enzyme activity Anas sp.
W9A mutant with 1.9% enzyme activity Anas sp.
W9F mutant with 11.3% enzyme activity Anas sp.
W9M mutant with 1.0% enzyme activity Anas sp.
W9R mutant with 3% enzyme activity Anas sp.
W9Y mutant with 49.5% enzyme activity Anas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.045
-
argininosuccinate wild type, 25 °C, 10 mM Tris-HCl, pH 7.5, 1 mM EDTA Anas sp.
0.069
-
argininosuccinate mutant W9R, 25 °C, 10 mM Tris-HCl, pH 7.5, 1 mM EDTA Anas sp.
0.083
-
argininosuccinate mutant TRUN1-8, 25 °C, 10 mM Tris-HCl, pH 7.5, 1 mM EDTA Anas sp.
0.105
-
argininosuccinate mutant TRUN1-9, 25 °C, 10 mM Tris-HCl, pH 7.5, 1 mM EDTA Anas sp.
0.106
-
argininosuccinate mutant W9Y, 25 °C, 10 mM Tris-HCl, pH 7.5, 1 mM EDTA Anas sp.
0.111
-
argininosuccinate mutant W9F, 25 °C, 10 mM Tris-HCl, pH 7.5, 1 mM EDTA Anas sp.
0.112
-
argininosuccinate mutant W9A, 25 °C, 10 mM Tris-HCl, pH 7.5, 1 mM EDTA Anas sp.
0.115
-
argininosuccinate mutant W9M, 25 °C, 10 mM Tris-HCl, pH 7.5, 1 mM EDTA Anas sp.
0.119
-
argininosuccinate 211-chimera, 25 °C, 10 mM Tris-HCl, pH 7.5, 1 mM EDTA Anas sp.
0.212
-
argininosuccinate mutant M9F/E115D, 25 °C, 10 mM Tris-HCl, pH 7.5, 1 mM EDTA Anas sp.
0.255
-
argininosuccinate mutant D115A, 25 °C, 10 mM Tris-HCl, pH 7.5, 1 mM EDTA Anas sp.
0.273
-
argininosuccinate mutant D115E, 25 °C, 10 mM Tris-HCl, pH 7.5, 1 mM EDTA Anas sp.
0.294
-
argininosuccinate mutant M9W/E115D, 25 °C, 10 mM Tris-HCl, pH 7.5, 1 mM EDTA Anas sp.
0.61
-
argininosuccinate mutant M9Y/E115D, 25 °C, 10 mM Tris-HCl, pH 7.5, 1 mM EDTA Anas sp.
2.724
-
argininosuccinate mutant M9W, 25 °C, 10 mM Tris-HCl, pH 7.5, 1 mM EDTA Anas sp.
3.159
-
argininosuccinate mutant M9W/V14S, 25 °C, 10 mM Tris-HCl, pH 7.5, 1 mM EDTA Anas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
omega-N-(L-arginino)succinate Anas sp.
-
L-arginine + fumarate
-
?

Organism

Organism UniProt Comment Textmining
Anas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Anas sp.

Source Tissue

Source Tissue Comment Organism Textmining
eye
-
Anas sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
argininosuccinate
-
Anas sp. fumarate + L-arginine
-
?
omega-N-(L-arginino)succinate
-
Anas sp. L-arginine + fumarate
-
?

Subunits

Subunits Comment Organism
homotetramer
-
Anas sp.

Synonyms

Synonyms Comment Organism
ASL delta2-crystallin Anas sp.