Literature summary for 4.3.2.1 extracted from

Chakraborty, A.R.; Davidson, A.; Howell, P.L.
Mutational analysis of amino acid residues involved in argininosuccinate lyase activity in duck delta II crystallin (1999), Biochemistry, 38, 2435-2443.

Search for more literature for 4.3.2.1

Protein Variants

Protein Variants	Comment	Organism
E296Q	retains only 0.001% residual activity	Anas platyrhynchos
N116L	no activity	Anas platyrhynchos
R115N	no activity	Anas platyrhynchos
S283A	no activity	Anas platyrhynchos
S384A	catalytic efficiency is decreased by an order of magnitude in comparison to wild-type delta II crystallin	Anas platyrhynchos
T161V	no activity	Anas platyrhynchos
Y323F	catalytic efficiency is decreased by an order of magnitude in comparison to wild-type delta II crystallin	Anas platyrhynchos
Y323I	no activity	Anas platyrhynchos

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.02	-	argininosuccinate	mutant enzyme S284A	Anas platyrhynchos
0.03	-	argininosuccinate	mutant enzyme R113N	Anas platyrhynchos
0.07	-	argininosuccinate	wild-type enzyme	Anas platyrhynchos
0.09	-	argininosuccinate	mutant enzyme S114A	Anas platyrhynchos
0.4	-	argininosuccinate	-	Anas platyrhynchos
1.7	-	argininosuccinate	mutant enzyme Y323F	Anas platyrhynchos

Organism

Organism	UniProt	Comment	Textmining
Anas platyrhynchos	-	wild type and mutant enzymes: S114A, R113N, S284A, Y323F, E296Q, r115N, N116L, T161V, S283A, and Y323I	-
Homo sapiens	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
2-(Nomega-L-arginino)succinate = fumarate + L-arginine	5 residues are essential for catalytic activity: R115, N116, T161, S283, and E296	Anas platyrhynchos	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
lens	-	Anas platyrhynchos	-

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.12	-	argininosuccinate	mutant enzyme S284A	Anas platyrhynchos
0.43	-	argininosuccinate	mutant enzyme Y323F	Anas platyrhynchos
0.62	-	argininosuccinate	mutant enzyme R113N	Anas platyrhynchos
2.42	-	argininosuccinate	mutant enzyme S114A	Anas platyrhynchos
3.28	-	argininosuccinate	-	Homo sapiens
4.9	-	argininosuccinate	wild-type enzyme	Anas platyrhynchos

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Release 2023.1 (January 2023)