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Literature summary for 4.3.1.B2 extracted from

  • Klem, T.J.; Chen, Y.; Davisson, V.J.
    Subunit interactions and glutamine utilization by Escherichia coli imidazole glycerol phosphate synthase (2001), J. Bacteriol., 182, 989-996.
No PubMed abstract available
Search for more literature for 4.3.1.B2

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Protein Variants

Protein Variants Comment Organism
C124R IGP synthases formed with Q123R mutant HisF subunit has no measurable activity with glutamine in vitro Escherichia coli
E46G IGP synthases formed with E46G mutant HisF subunit shows 2800fold reduction in the kcat/Km ratio for glutamine Escherichia coli
Q123R IGP synthases formed with Q123R mutant HisF subunit has no measurable activity with glutamine in vitro Escherichia coli
R5H IGP synthases formed with R5H mutant HisF subunit shows 1500fold reduction in the kcat/Km ratio for glutamine Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0125
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, IGP synthase enzyme with a E46G mutant subunit HisF Escherichia coli
0.0175
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, IGP synthase enzyme with a Q123R mutant subunit HisF Escherichia coli
0.0218
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, IGP synthase enzyme with a C124R mutant subunit HisF Escherichia coli
0.0426
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF Escherichia coli
1 1.2 NH4+ pH 8.0, 30°C, IGP synthase enzyme with a E46G mutant subunit HisF Escherichia coli
1 4.5 L-glutamine pH 8.0, 30°C, IGP synthase enzyme with a E46G mutant subunit HisF Escherichia coli
5.4
-
 L-glutamine pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF Escherichia coli
7.4
-
 NH4+ pH 8.0, 30°C, IGP synthase enzyme with a C124R mutant subunit HisF Escherichia coli
13.4
-
 NH4+ pH 8.0, 30°C, IGP synthase enzyme with a Q123R mutant subunit HisF Escherichia coli
14.3
-
 NH4+ pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamine + N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate Escherichia coli
-
 L-glutamate + D-erythro-1-(imidazol-4-yl)glycerol phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P60595 and P60664 P60595: IGP synthase glutamine amidotransferase subunit, P60664: IGP synthase cyclase subunit
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate
-
 Escherichia coli L-glutamate + D-erythro-1-(imidazol-4-yl)glycerol phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
 ?
NH4+ + N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate
-
 Escherichia coli D-erythro-1-(imidazol-4-yl)glycerol phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
 ?

Subunits

Subunits Comment Organism
heterodimer
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.13
-
 L-glutamine pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF Escherichia coli
0.19
-
 L-glutamine pH 8.0, 30°C, IGP synthase enzyme with a E46G mutant subunit HisF Escherichia coli
3.7
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, IGP synthase enzyme with a E46G mutant subunit HisF Escherichia coli
4.3
-
 NH4+ pH 8.0, 30°C, IGP synthase enzyme with a C124R mutant subunit HisF Escherichia coli
4.4
-
 NH4+ pH 8.0, 30°C, IGP synthase enzyme enzyme with a E46G mutant subunit HisF Escherichia coli
5.2
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, IGP synthase enzyme with a C124R mutant subunit HisF Escherichia coli
5.2
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF Escherichia coli
5.3
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, IGP synthase enzyme with a Q123R mutant subunit HisF Escherichia coli
5.7
-
 NH4+ pH 8.0, 30°C, IGP synthase enzyme with a Q123R mutant subunit HisF Escherichia coli
5.7
-
 NH4+ pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.013
-
 L-glutamine pH 8.0, 30°C, IGP synthase enzyme with a E46G mutant subunit HisF Escherichia coli
0.024
-
 L-glutamine pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF Escherichia coli
0.392
-
 NH4+ pH 8.0, 30°C, IGP synthase enzyme with a E46G mutant subunit HisF Escherichia coli
0.399
-
 NH4+ pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF Escherichia coli
0.425
-
 NH4+ pH 8.0, 30°C, IGP synthase enzyme with a Q123R mutant subunit HisF Escherichia coli
0.581
-
 NH4+ pH 8.0, 30°C, IGP synthase enzyme with a C124R mutant subunit HisF Escherichia coli
120
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF Escherichia coli
240
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, IGP synthase enzyme with a C124R mutant subunit HisF Escherichia coli
300
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, IGP synthase enzyme with a E46G mutant subunit HisF Escherichia coli
300
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, IGP synthase enzyme with a Q123R mutant subunit HisF Escherichia coli