Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D362A | mutant shows highly decreased kcat compared to wild-type, mutant undergoes quicker inactivation compared to wild-type | Escherichia coli |
D362E | mutant shows highly decreased kcat compared to wild-type | Escherichia coli |
D362N | mutant shows highly decreased kcat compared to wild-type | Escherichia coli |
E287A | mutant shows highly decreased kcat compared to wild-type, mutant undergoes quicker inactivation compared to wild-type | Escherichia coli |
E287D | mutant shows highly decreased kcat compared to wild-type | Escherichia coli |
E287H | mutant shows highly decreased kcat compared to wild-type | Escherichia coli |
E287Q | mutant shows highly decreased kcat compared to wild-type | Escherichia coli |
N193A | mutant shows highly decreased kcat compared to wild-type, mutant undergoes quicker inactivation compared to wild-type | Escherichia coli |
N193D | mutant shows decreased kcat compared to wild-type but mutant retains partial activity, Km (ethanolamine) increased compared to wild-type | Escherichia coli |
Q162A | mutant shows highly decreased kcat compared to wild-type, mutant undergoes quicker inactivation compared to wild-type | Escherichia coli |
Q162E | mutant shows decreased kcat compared to wild-type but mutant retains partial activity, Km (ethanolamine) increased compared to wild-type | Escherichia coli |
Q162H | mutant shows decreased kcat compared to wild-type but mutant retains partial activity, Km (ethanolamine) increased compared to wild-type | Escherichia coli |
Q162K | mutant shows highly decreased kcat compared to wild-type | Escherichia coli |
R160A | mutant shows highly decreased kcat compared to wild-type, mutant undergoes quicker inactivation compared to wild-type | Escherichia coli |
R160K | mutant shows decreased kcat compared to wild-type but mutant retains partial activity, Km (ethanolamine) increased compared to wild-type | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0033 | - |
ethanolamine | run 2, pH 8, 37°C, wild-type | Escherichia coli | |
0.21 | - |
ethanolamine | run 2, pH 8, 37°C, mutant N193D | Escherichia coli | |
0.21 | - |
ethanolamine | run 2, pH 8, 37°C, mutant R160K | Escherichia coli | |
0.22 | - |
ethanolamine | run 2, pH 8, 37°C, mutant Q162H | Escherichia coli | |
0.73 | - |
ethanolamine | run 2, pH 8, 37°C, mutant Q162E | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethanolamine | - |
Escherichia coli | acetaldehyde + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EAL | - |
Escherichia coli |
ethanolamine ammonia-lyase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.036 | - |
ethanolamine | run 1, pH 8, 37°C, mutant E287A | Escherichia coli | |
0.038 | - |
ethanolamine | run 2, pH 8, 37°C, mutant D362N | Escherichia coli | |
0.051 | - |
ethanolamine | run 2, pH 8, 37°C, mutant Q162K | Escherichia coli | |
0.066 | - |
ethanolamine | run 2, pH 8, 37°C, mutant E287H | Escherichia coli | |
0.085 | - |
ethanolamine | run 1, pH 8, 37°C, mutant D362A | Escherichia coli | |
0.086 | - |
ethanolamine | run 2, pH 8, 37°C, mutant E287Q | Escherichia coli | |
0.67 | - |
ethanolamine | run 2, pH 8, 37°C, mutant D362E | Escherichia coli | |
1 | - |
ethanolamine | run 1, pH 8, 37°C, mutant R160A | Escherichia coli | |
1.3 | - |
ethanolamine | run 1, pH 8, 37°C, mutant N193A | Escherichia coli | |
2.1 | - |
ethanolamine | run 2, pH 8, 37°C, mutant E287D | Escherichia coli | |
3 | 6 | ethanolamine | run 2, pH 8, 37°C, mutant N193D | Escherichia coli | |
6 | - |
ethanolamine | run 2, pH 8, 37°C, mutant Q162H | Escherichia coli | |
20 | - |
ethanolamine | run 1, pH 8, 37°C, mutant Q162A | Escherichia coli | |
79 | - |
ethanolamine | run 2, pH 8, 37°C, mutant R160K | Escherichia coli | |
110 | - |
ethanolamine | run 2, pH 8, 37°C, mutant Q162E | Escherichia coli | |
440 | - |
ethanolamine | run 2, pH 8, 37°C, wild-type | Escherichia coli | |
770 | - |
ethanolamine | run 1, pH 8, 37°C, wild-type | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
adenosylcobalamin | - |
Escherichia coli |