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Literature summary for 4.3.1.7 extracted from
- The structural model of Salmonella typhimurium ethanolamine ammonia-lyase directs a rational approach to the assembly of the functional [(EutB-EutC)2]3 oligomer from isolated subunits (2013), Biochemistry, 52, 1419-1428.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| tris-(2-carboxyethyl) phosphine | leads to 25fold increase in kcat | Salmonella enterica subsp. enterica serovar Typhimurium |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed as a His-taggd fusion protein | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.022 | - |
ethanolamine | reconstituted EAL (from EutB and EutC subunits), pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.0316 | - |
ethanolamine | purified EAL, pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
SDS-PAGE, EutC monomer | Salmonella enterica subsp. enterica serovar Typhimurium |
| 48000 | - |
SDS-PAGE, EutB monomer | Salmonella enterica subsp. enterica serovar Typhimurium |
| 494000 | - |
native PAGE, EAL oligomer | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica subsp. enterica serovar Typhimurium | P19264 and P19265 | heavy chain: P19264, light chain: P19265 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ethanolamine | - |
Salmonella enterica subsp. enterica serovar Typhimurium | acetaldehyde + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| oligomer | EutB and EutC monomers form the EutB-EutC heterodimer. Two heterodimers form a (EutB-EutC)2 homodimer, and three (EutB-EutC)2 homodimers form the biologically active oligomer. The model predicts that (EutB2)3 oligomer assembly occurs from isolated EutB, and that this hexameric structure templates the formation of the complete, native [(EutB-EutC)2]3 oligomer | Salmonella enterica subsp. enterica serovar Typhimurium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EAL | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
| ethanolamine ammonia-lyase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 16.7 | - |
ethanolamine | reconstituted EAL (from EutB and EutC subunits), pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 22.7 | - |
ethanolamine | purified EAL, pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 720 | - |
ethanolamine | purified EAL, pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 730 | - |
ethanolamine | reconstituted EAL (from EutB and EutC subunits), pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
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