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Literature summary for 4.3.1.32 extracted from

  • Graham, D.E.; Xu, H.; White, R.H.
    Identification of the 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase required for coenzyme F(420) biosynthesis (2003), Arch. Microbiol., 180, 455-464 .
    View publication on PubMed
Search for more literature for 4.3.1.32

Activating Compound

Activating Compound Comment Organism Structure
Dithionite after activation with Fe2+, HS-, S-adenosyl-L-methionine and dithionite, activity increases almost 10fold Mycolicibacterium smegmatis

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ after activation with Fe2+, HS-, S-adenosyl-L-methionine and dithionite, activity increases almost 10fold Mycolicibacterium smegmatis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 SDS-PAGE Methanocaldococcus jannaschii
88000
-
 SDS-PAGE Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil + S-adenosyl-L-methionine Mycolicibacterium smegmatis the reaction is catalzed by the bifunctional FO synthase (EC 2.5.1.147/EC 4.3.1.32) 7,8-didemethyl-8-hydroxy-5-deazariboflavin + NH3 + L-methionine + 5'-deoxyadenosine
-
 ?

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q57888
-
-
Methanocaldococcus jannaschii DSM 2661 Q57888
-
-
Mycolicibacterium smegmatis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil + S-adenosyl-L-methionine
-
 Methanocaldococcus jannaschii 7,8-didemethyl-8-hydroxy-5-deazariboflavin + NH3 + L-methionine + 5'-deoxyadenosine
-
 ?
5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil + S-adenosyl-L-methionine the reaction is catalzed by the bifunctional FO synthase (EC 2.5.1.147/EC 4.3.1.32) Mycolicibacterium smegmatis 7,8-didemethyl-8-hydroxy-5-deazariboflavin + NH3 + L-methionine + 5'-deoxyadenosine
-
 ?
5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil + S-adenosyl-L-methionine
-
 Methanocaldococcus jannaschii DSM 2661 7,8-didemethyl-8-hydroxy-5-deazariboflavin + NH3 + L-methionine + 5'-deoxyadenosine
-
 ?

Synonyms

Synonyms Comment Organism
cofG
-
 Methanocaldococcus jannaschii
fbiC gene name, bifunctional FO synthase (EC 2.5.1.147/EC 4.3.1.32). In the actinomycetes, cofG and cofH are fused, encoding a single FbiC polypeptide Mycolicibacterium smegmatis
MjCofG
-
 Methanocaldococcus jannaschii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 activity is not significantly reduced during incubations at 70°C Mycolicibacterium smegmatis

Expression

Organism Comment Expression
Mycolicibacterium smegmatis cloning of the fused Mycobacterium smegmatis fbiC gene and heterologous expression of the bifunctional MsFbiC in Escherichia coli additional information
Methanocaldococcus jannaschii heterologous expression in Escherichia coli and functional characterization in vitro. MjCofG is substantially soluble in cell-free extract additional information

General Information

General Information Comment Organism
metabolism the enzyme is involved in the production of 7,8-didemethyl-8-hydroxy-5-deazariboflavin, the precursor of the redox cofactor coenzyme F420. In the actinomycetes, cofG and cofH are fused, encoding a single FbiC polypeptide, the bifunctional FO synthase (EC 2.5.1.147/EC 4.3.1.32) Mycolicibacterium smegmatis
metabolism the enzyme produces the 7,8-didemethyl-8-hydroxy-5-deazariboflavin precursor of the redox cofactor coenzyme F420. The precursor of the redox cofactor coenzyme F420 Methanocaldococcus jannaschii