Any feedback?
Literature summary for 4.3.1.3 extracted from
- Characterization of the active site of histidine ammonia-lyase from Pseudomonas putida (2001), Eur. J. Biochem., 268, 6011-6019.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pseudomonas putida |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C273A | 20% of wild-type kcat | Pseudomonas putida |
| C273A/E414A | no activity | Pseudomonas putida |
| C273A/E414Q | 0.06% of wild-type kcat | Pseudomonas putida |
| C273A/F329A | 0.2% of wild-type kcat | Pseudomonas putida |
| C273A/H83L | no activity | Pseudomonas putida |
| C273A/N195A | 0.02% of wild-type kcat | Pseudomonas putida |
| C273A/Q277A | 0.2% of wild-type kcat | Pseudomonas putida |
| C273A/R283I | 0.9% of wild-type kcat | Pseudomonas putida |
| C273A/R283K | 0.01% of wild-type kcat | Pseudomonas putida |
| C273A/Y280F | 0.4% of wild-type kcat | Pseudomonas putida |
| C273A/Y53F | no activity | Pseudomonas putida |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| L-cysteine | 10 mM, irreversible inactivation | Pseudomonas putida |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.9 | - |
L-histidine | 25°C, pH 9.3 | Pseudomonas putida | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-histidine | Pseudomonas putida | - |
urocanate + NH3 | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | P21310 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant histidase | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-histidine | - |
Pseudomonas putida | urocanate + NH3 | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HAL | - |
Pseudomonas putida |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 86 | - |
L-histidine | 25°C, pH 9.3 | Pseudomonas putida | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
