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Literature summary for 4.3.1.3 extracted from

  • Hernandez, D.; Phillips, A.T.
    Purification and Characterization of Pseudomonas putida histidine ammonia-lyase expressed in Escherichia coli (1993), Protein Expr. Purif., 4, 473-478.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
CN- KCN Pseudomonas putida
CN- KCN Pseudomonas sp.
D-histidine cloned enzyme, reversible competitive Pseudomonas putida
EDTA 0.005 mM, 75% decrease of activity Pseudomonas putida
EDTA
-
Pseudomonas sp.
L-cysteine competitive, cloned enzyme Pseudomonas putida
L-cysteine
-
Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3
-
L-histidine native enzyme, pH 9.0 Pseudomonas putida
5.8
-
L-histidine cloned enzyme, pH 9.0 Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Pseudomonas putida
Ni2+
-
Pseudomonas putida
Zn2+
-
Pseudomonas sp.
Zn2+
-
Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53700
-
4 * 53700, calculation from sequence of amino acid Pseudomonas putida
220000
-
gel filtration Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
ATCC 12633
-
Pseudomonas putida
-
PRS1
-
Pseudomonas putida PRS1
-
PRS1
-
Pseudomonas sp.
-
ATCC 11,299b
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Pseudomonas sp.
additional information
-
-
Pseudomonas putida
70.4
-
cloned enzyme Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-histidine natural substrate Pseudomonas sp. urocanate + NH3
-
?
L-histidine natural substrate Pseudomonas putida urocanate + NH3
-
?
L-histidine non-oxidative deamination Pseudomonas putida urocanate + NH3
-
?
L-histidine natural substrate Pseudomonas putida PRS1 urocanate + NH3
-
?
L-histidine non-oxidative deamination Pseudomonas putida PRS1 urocanate + NH3
-
?
L-histidine methyl ester cloned enzyme Pseudomonas putida urocanate methyl ester cloned enzyme ?
L-histidine methyl ester cloned enzyme Pseudomonas putida PRS1 urocanate methyl ester cloned enzyme ?

Subunits

Subunits Comment Organism
tetramer
-
Pseudomonas sp.
tetramer 4 * 53700, calculation from sequence of amino acid Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
255
-
L-histidine
-
Pseudomonas putida