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Literature summary for 4.3.1.3 extracted from
- L-histidine ammonia-lyase in rat liver. I. Purification and general characteristics (1974), J. Biochem., 75, 139-152.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | activates at concentrations up to 10 mM | Rattus norvegicus |  |
| GSH | - |
Cavia porcellus | |
| GSH | activates | Rattus norvegicus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | inhibition at concentrations above 10 mM, reversed by addition of metal ion | Rattus norvegicus | |
| dithiothreitol | - |
Cavia porcellus | |
| dithiothreitol | - |
Rattus norvegicus | |
| EDTA | - |
Cavia porcellus | |
| EDTA | - |
Rattus norvegicus | |
| additional information | - |
Rattus norvegicus | |
| NaBH4 | - |
Rattus norvegicus | |
| Nitromethane | - |
Rattus norvegicus | |
| p-mercuribenzoate | 0.01 mM, complete inhibition, partially reversed by addition of histidine | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
L-histidine | - |
Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates at a concentration of 0.1 mM | Rattus norvegicus | |
| Cd2+ | activates at a concentration of 0.1 mM | Rattus norvegicus | |
| Mg2+ | activates at a concentration of 0.1 mM | Rattus norvegicus | |
| Mn2+ | activates at a concentration of 0.1 mM | Rattus norvegicus | |
| Ni2+ | activates at a concentration of 0.1 mM | Rattus norvegicus | |
| Zn2+ | - |
Cavia porcellus | |
| Zn2+ | activates at a concentration of 0.1 mM | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 190000 | - |
sedimentation equilibrium | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cavia porcellus | - |
- |
- |
| Rattus norvegicus | - |
female Spargue-Dawley | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Cavia porcellus | - |
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Cavia porcellus |
| additional information | - |
- |
Rattus norvegicus |
| 6.8 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, 24 h, pH 3.0 or pH 12.0, complete loss of activity | Rattus norvegicus |
| 0°C, 24 h, pH 7.0-10.0, enzyme retains full activity | Rattus norvegicus |
| 0°C, complete loss of activity within 1 week | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-histidine | natural substrate | Cavia porcellus | urocanate + NH3 | - |
? | |
| L-histidine | natural substrate | Rattus norvegicus | urocanate + NH3 | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.8 | 9 | - |
Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | pH 7.0: 10% of maximal activity, pH 10: about 50% of maximal activity | Rattus norvegicus |
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