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Literature summary for 4.3.1.25 extracted from

  • Xue, Z.; McCluskey, M.; Cantera, K.; Ben-Bassat, A.; Sariaslani, F.S.; Huang, L.
    Improved production of p-hydroxycinnamic acid from tyrosine using a novel thermostable phenylalanine/tyrosine ammonia lyase enzyme (2007), Enzyme Microb. Technol., 42, 58-64.
No PubMed abstract available
Search for more literature for 4.3.1.25

Cloned(Commentary)

Cloned (Comment) Organism
controlled expression in Escherichia coli to mitigate product toxicity Rhodotorula glutinis
controlled expression in Escherichia coli to mitigate product toxicity Phanerodontia chrysosporium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.044
-
 L-tyrosine pH 9.5, 35°C Phanerodontia chrysosporium
0.0677
-
 L-tyrosine pH 9.5 Rhodotorula glutinis
0.126
-
 L-phenylalanine pH 9.5 Rhodotorula glutinis
0.161
-
 L-phenylalanine pH 9.5, 35°C Phanerodontia chrysosporium
0.183
-
 L-phenylalanine pH 8.5 Rhodotorula glutinis
0.615
-
 L-tyrosine pH 8.5 Rhodotorula glutinis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 x * 70000, SDS-PAGE Phanerodontia chrysosporium

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium
-
-
-
Rhodotorula glutinis
-
-
-

Storage Stability

Storage Stability Organism
-80°C, 50 mM Tris, pH 8.5, 25% glycerol, stable Phanerodontia chrysosporium
4°C, 50 mM Tris, pH 8.5, stable for at least a month Phanerodontia chrysosporium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine
-
 Rhodotorula glutinis (E)-cinnamate + NH3
-
 ?
L-phenylalanine
-
 Phanerodontia chrysosporium (E)-cinnamate + NH3
-
 ?
L-tyrosine
-
 Rhodotorula glutinis p-hydroxycinnamic acid + NH3
-
 ?
L-tyrosine
-
 Phanerodontia chrysosporium p-hydroxycinnamic acid + NH3
-
 ?
additional information no substrate: L-histidine Phanerodontia chrysosporium ?
-
 ?

Subunits

Subunits Comment Organism
? x * 70000, SDS-PAGE Phanerodontia chrysosporium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 3 h, no loss of activity, 4 h, 72% residual activity Phanerodontia chrysosporium
60
-
 4 h, 10% residual activity Rhodotorula glutinis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.53
-
 L-tyrosine pH 8.5 Rhodotorula glutinis
0.93
-
 L-tyrosine pH 9.5 Rhodotorula glutinis
1.3
-
 L-tyrosine pH 9.5, 35°C Phanerodontia chrysosporium
1.53
-
 L-phenylalanine pH 9.5 Rhodotorula glutinis
1.6
-
 L-phenylalanine pH 8.5 Rhodotorula glutinis
3.3
-
 L-phenylalanine pH 9.5, 35°C Phanerodontia chrysosporium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 35°C Phanerodontia chrysosporium

pH Range

pH Minimum pH Maximum Comment Organism
8 9.5 above 88% of maximum activity Phanerodontia chrysosporium
10
-
 80% of maximum activity Phanerodontia chrysosporium
12
-
 12% of maximum activity Phanerodontia chrysosporium