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Literature summary for 4.3.1.25 extracted from

  • Adachi, O.; Matsushita, K.; Shinagawa, E.; Ameyama, M.
    Crystallization and properties of L-phenylalanine ammonia-lyase from Rhodosporidium toruloides (1990), Agric. Biol. Chem., 54, 2839-2843.
No PubMed abstract available

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Rhodotorula toruloides

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ HgCl2 Rhodotorula toruloides
p-chloromercuribenzoate
-
Rhodotorula toruloides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
L-Tyr
-
Rhodotorula toruloides
0.29
-
L-Phe
-
Rhodotorula toruloides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
2 * 80000, SDS-PAGE Rhodotorula toruloides
165000
-
gel filtration, equilibrium sedimentation Rhodotorula toruloides

Organism

Organism UniProt Comment Textmining
Rhodotorula toruloides
-
IFO 0559
-

Purification (Commentary)

Purification (Comment) Organism
-
Rhodotorula toruloides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Rhodotorula toruloides

Storage Stability

Storage Stability Organism
4°C, stable for at least 1 month Rhodotorula toruloides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Phe
-
Rhodotorula toruloides (E)-cinnamate + NH3
-
?
L-tyrosine L-Tyr Rhodotorula toruloides p-coumarate + NH3
-
?
L-tyrosine 50% of the activity with L-Phe Rhodotorula toruloides p-coumarate + NH3
-
?

Subunits

Subunits Comment Organism
dimer 2 * 80000, SDS-PAGE Rhodotorula toruloides

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
L-Phe increases thermostability Rhodotorula toruloides
50
-
10 min, stable Rhodotorula toruloides
70
-
10 min, complete inactivation Rhodotorula toruloides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Rhodotorula toruloides