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Literature summary for 4.3.1.25 extracted from

  • Abell, C.W.; Shen, R.S.
    Phenylalanine ammonia-lyase from the yeast Rhodotorula glutinis (1987), Methods Enzymol., 142, 242-249.
    View publication on PubMed
Search for more literature for 4.3.1.25

Application

Application Comment Organism
analysis rapid quantization of Phe and Tyr in plasma and serum from subjects with phenylketonuria Rhodotorula glutinis

Inhibitors

Inhibitors Comment Organism Structure
4-coumarate
-
 Rhodotorula glutinis
Cinnamic acid trans-cinnamic acid Rhodotorula glutinis
D-Phe
-
 Rhodotorula glutinis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 L-Tyr
-
 Rhodotorula glutinis
0.25
-
 L-Phe
-
 Rhodotorula glutinis
4.2
-
 trans-cinnamate
-
 Rhodotorula glutinis
3170
-
 NH3
-
 Rhodotorula glutinis

Organism

Organism UniProt Comment Textmining
Rhodotorula glutinis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rhodotorula glutinis

Reaction

Reaction Comment Organism Reaction ID
L-phenylalanine = trans-cinnamate + NH3 ordered uni-bi reaction sequence Rhodotorula glutinis
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Rhodotorula glutinis

Storage Stability

Storage Stability Organism
-60°C, enzyme concentration 20-40 mg/ml, stable for 6 months Rhodotorula glutinis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Phe
-
 Rhodotorula glutinis (E)-cinnamate + NH3
-
 r
L-tyrosine L-Tyr Rhodotorula glutinis p-coumarate + NH3
-
 ?
trans-cinnamate + NH3
-
 Rhodotorula glutinis L-Phe
-
 r