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Literature summary for 4.3.1.24 extracted from

  • Bencze, L.C.; Filip, A.; Banoczi, G.; Tosa, M.I.; Irimie, F.D.; Gellert, A.; Poppe, L.; Paizs, C.
    Expanding the substrate scope of phenylalanine ammonia-lyase from Petroselinum crispum towards styrylalanines (2017), Org. Biomol. Chem., 15, 3717-3727 .
    View publication on PubMed
Search for more literature for 4.3.1.24

Protein Variants

Protein Variants Comment Organism
F137V the mutant enzyme can transform L-styrylalanine with comparable activity to that of the wild-type enzyme with L-phenylalanine. Ammonia elimination from L-styrylalanine by the non-mutated wild-type enzyme takes place with a 777fold lower kcat/KM value than the deamination of the natural substrate, L-phenylalanine. The mutant enzyme shows enhanced catalytic efficiency in the ammonia elimination reaction of several racemic styrylalanine derivatives Petroselinum crispum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0783
-
 rac-(E)-2-amino-5-(4-chlorophenyl)pent-4-enoic acid pH 8.8, 30°C, mutant enzyme F137V Petroselinum crispum
0.083
-
 L-phenylalanine pH 8.8, 30°C, wild-type enzyme Petroselinum crispum
0.086
-
 L-phenylalanine pH 8.8, 30°C, mutant enzyme F137V Petroselinum crispum
0.154
-
 rac-(E)-2-amino-5-(4-chlorophenyl)pent-4-enoic acid pH 8.8, 30°C, wild-type enzyme Petroselinum crispum
0.186
-
 L-styrylalanine pH 8.8, 30°C, mutant enzyme F137V Petroselinum crispum
0.201
-
 rac-(E)-2-amino-5-phenylpent-4-enoic acid pH 8.8, 30°C, mutant enzyme F137V Petroselinum crispum
0.395
-
 rac-(E)-2-amino-5-phenylpent-4-enoic acid pH 8.8, 30°C, wild-type enzyme Petroselinum crispum
1.173
-
 L-styrylalanine pH 8.8, 30°C, mutant enzyme F137A Petroselinum crispum
1.732
-
 L-phenylalanine pH 8.8, 30°C, mutant enzyme F137A Petroselinum crispum
4.12
-
 L-styrylalanine pH 8.8, 30°C, mutant enzyme F137G Petroselinum crispum
4.384
-
 L-styrylalanine pH 8.8, 30°C, wild-type enzyme Petroselinum crispum
4.969
-
 L-phenylalanine pH 8.8, 30°C, mutant enzyme F137G Petroselinum crispum

Organism

Organism UniProt Comment Textmining
Petroselinum crispum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine
-
 Petroselinum crispum trans-cinnamate + NH3
-
 ?
L-styrylalanine
-
 Petroselinum crispum (styryl)acrylic acid + NH3
-
 ?
rac-(E)-2-amino-5-(4-chlorophenyl)pent-4-enoic acid
-
 Petroselinum crispum (2E,4E)-5-(4-chlorophenyl)penta-2,4-dienoic acid + NH3
-
 ?
rac-(E)-2-amino-5-phenylpent-4-enoic acid
-
 Petroselinum crispum (2E,4E)-5-phenylpenta-2,4-dienoic acid + NH3
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Petroselinum crispum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00024
-
 rac-(E)-2-amino-5-(4-chlorophenyl)pent-4-enoic acid pH 8.8, 30°C, wild-type enzyme Petroselinum crispum
0.0062
-
 rac-(E)-2-amino-5-phenylpent-4-enoic acid pH 8.8, 30°C, wild-type enzyme Petroselinum crispum
0.0345
-
 L-styrylalanine pH 8.8, 30°C, mutant enzyme F137G Petroselinum crispum
0.0471
-
 L-styrylalanine pH 8.8, 30°C, wild-type enzyme Petroselinum crispum
0.052
-
 L-phenylalanine pH 8.8, 30°C, mutant enzyme F137G Petroselinum crispum
0.0786
-
 rac-(E)-2-amino-5-(4-chlorophenyl)pent-4-enoic acid pH 8.8, 30°C, mutant enzyme F137V Petroselinum crispum
0.132
-
 L-styrylalanine pH 8.8, 30°C, mutant enzyme F137A Petroselinum crispum
0.173
-
 L-phenylalanine pH 8.8, 30°C, mutant enzyme F137V Petroselinum crispum
0.276
-
 rac-(E)-2-amino-5-phenylpent-4-enoic acid pH 8.8, 30°C, mutant enzyme F137V Petroselinum crispum
0.283
-
 L-phenylalanine pH 8.8, 30°C, mutant enzyme F137A Petroselinum crispum
0.422
-
 L-styrylalanine pH 8.8, 30°C, mutant enzyme F137V Petroselinum crispum
0.694
-
 L-phenylalanine pH 8.8, 30°C, wild-type enzyme Petroselinum crispum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
 assay at Petroselinum crispum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0015
-
 rac-(E)-2-amino-5-(4-chlorophenyl)pent-4-enoic acid pH 8.8, 30°C, wild-type enzyme Petroselinum crispum
0.0083
-
 L-styrylalanine pH 8.8, 30°C, mutant enzyme F137G Petroselinum crispum
0.0104
-
 L-phenylalanine pH 8.8, 30°C, mutant enzyme F137G Petroselinum crispum
0.0107
-
 L-styrylalanine pH 8.8, 30°C, wild-type enzyme Petroselinum crispum
0.0156
-
 rac-(E)-2-amino-5-phenylpent-4-enoic acid pH 8.8, 30°C, wild-type enzyme Petroselinum crispum
0.1125
-
 L-styrylalanine pH 8.8, 30°C, mutant enzyme F137A Petroselinum crispum
0.163
-
 L-phenylalanine pH 8.8, 30°C, mutant enzyme F137A Petroselinum crispum
1.004
-
 rac-(E)-2-amino-5-(4-chlorophenyl)pent-4-enoic acid pH 8.8, 30°C, mutant enzyme F137V Petroselinum crispum
1.373
-
 rac-(E)-2-amino-5-phenylpent-4-enoic acid pH 8.8, 30°C, mutant enzyme F137V Petroselinum crispum
2.011
-
 L-phenylalanine pH 8.8, 30°C, mutant enzyme F137V Petroselinum crispum
2.269
-
 L-styrylalanine pH 8.8, 30°C, mutant enzyme F137V Petroselinum crispum
8.361
-
 L-phenylalanine pH 8.8, 30°C, wild-type enzyme Petroselinum crispum