Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.3.1.24 extracted from

  • Jaliani, H.Z.; Farajnia, S.; Mohammadi, S.A.; Barzegar, A.; Talebi, S.
    Engineering and kinetic stabilization of the therapeutic enzyme Anabeana variabilis phenylalanine ammonia lyase (2013), Appl. Biochem. Biotechnol., 171, 1805-1818.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Trichormus variabilis

Protein Variants

Protein Variants Comment Organism
Q292C/C565S Km and kcat similar to wild-type, specific activity increased in mutant compared to wild-type, T1/2 increased in mutant compared to wild-type Trichormus variabilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
L-phenylalanine wild-type, pH and temperature not specified in the publication Trichormus variabilis
0.08
-
L-phenylalanine mutant Q292C/C565S, pH and temperature not specified in the publication Trichormus variabilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62500
-
SDS-PAGE Trichormus variabilis

Organism

Organism UniProt Comment Textmining
Trichormus variabilis Q3M5Z3
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Trichormus variabilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2
-
wild-type, pH and temperature not specified in the publication Trichormus variabilis
1.8
-
mutant Q292C/C565S, pH and temperature not specified in the publication Trichormus variabilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine
-
Trichormus variabilis (E)-cinnamate + NH3
-
?

Synonyms

Synonyms Comment Organism
AvPAL
-
Trichormus variabilis
phenylalanine ammonia lyase
-
Trichormus variabilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
62
-
T1/2 at 62°C: 29 h (wild-type), 44 h (mutant Q292C/C565S) Trichormus variabilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.9
-
L-phenylalanine mutant Q292C/C565S, pH and temperature not specified in the publication Trichormus variabilis
4.2
-
L-phenylalanine wild-type, pH and temperature not specified in the publication Trichormus variabilis

pH Range

pH Minimum pH Maximum Comment Organism
7.5 8.5 highest activity of wild-type and mutant enzyme Trichormus variabilis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
49
-
L-phenylalanine mutant Q292C/C565S, pH and temperature not specified in the publication Trichormus variabilis
70
-
L-phenylalanine wild-type, pH and temperature not specified in the publication Trichormus variabilis