Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Trichormus variabilis |
Protein Variants | Comment | Organism |
---|---|---|
Q292C/C565S | Km and kcat similar to wild-type, specific activity increased in mutant compared to wild-type, T1/2 increased in mutant compared to wild-type | Trichormus variabilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
L-phenylalanine | wild-type, pH and temperature not specified in the publication | Trichormus variabilis | |
0.08 | - |
L-phenylalanine | mutant Q292C/C565S, pH and temperature not specified in the publication | Trichormus variabilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62500 | - |
SDS-PAGE | Trichormus variabilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichormus variabilis | Q3M5Z3 | - |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Trichormus variabilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.2 | - |
wild-type, pH and temperature not specified in the publication | Trichormus variabilis |
1.8 | - |
mutant Q292C/C565S, pH and temperature not specified in the publication | Trichormus variabilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine | - |
Trichormus variabilis | (E)-cinnamate + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AvPAL | - |
Trichormus variabilis |
phenylalanine ammonia lyase | - |
Trichormus variabilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
62 | - |
T1/2 at 62°C: 29 h (wild-type), 44 h (mutant Q292C/C565S) | Trichormus variabilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.9 | - |
L-phenylalanine | mutant Q292C/C565S, pH and temperature not specified in the publication | Trichormus variabilis | |
4.2 | - |
L-phenylalanine | wild-type, pH and temperature not specified in the publication | Trichormus variabilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | highest activity of wild-type and mutant enzyme | Trichormus variabilis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
49 | - |
L-phenylalanine | mutant Q292C/C565S, pH and temperature not specified in the publication | Trichormus variabilis | |
70 | - |
L-phenylalanine | wild-type, pH and temperature not specified in the publication | Trichormus variabilis |