Application | Comment | Organism |
---|---|---|
analysis | immobilization of phenylalanine ammonia-lyase into gelatin on polyester films to determine phenylalanine in urine for the prediagnosis of phenylketonuria. Immobilized enzyme retaines 100% apparent activity after 30 days and as much as 75% of activity is retained after 2 months. The method is sufficiently sensitive to determine the phenylalanine concentration in phenylketonuric infants' urine | Rhodotorula glutinis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14.2 | - |
L-Phe | free enzyme, pH 8.7, 30°C | Rhodotorula glutinis | |
64.9 | - |
L-Phe | immobilized enzyme, pH 8.7, 30°C | Rhodotorula glutinis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodotorula glutinis | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Rhodotorula glutinis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.14 | - |
immobilized enzyme, pH 8.7, 30°C | Rhodotorula glutinis |
0.39 | - |
free enzyme, pH 8.7, 30°C | Rhodotorula glutinis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Phe | - |
Rhodotorula glutinis | (E)-cinnamate + NH3 | - |
? |