Any feedback?
Literature summary for 4.3.1.24 extracted from
- Properties of immobilized phenylalanine ammonia lyase and investigation of its use for the prediagnosis of phenylketonuria (2010), Turk. J. Biochem., 35, 58-62.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | immobilization of phenylalanine ammonia-lyase into gelatin on polyester films to determine phenylalanine in urine for the prediagnosis of phenylketonuria. Immobilized enzyme retaines 100% apparent activity after 30 days and as much as 75% of activity is retained after 2 months. The method is sufficiently sensitive to determine the phenylalanine concentration in phenylketonuric infants' urine | Rhodotorula glutinis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 14.2 | - |
L-Phe | free enzyme, pH 8.7, 30°C | Rhodotorula glutinis |  |
| 64.9 | - |
L-Phe | immobilized enzyme, pH 8.7, 30°C | Rhodotorula glutinis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodotorula glutinis | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Rhodotorula glutinis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.14 | - |
immobilized enzyme, pH 8.7, 30°C | Rhodotorula glutinis |
| 0.39 | - |
free enzyme, pH 8.7, 30°C | Rhodotorula glutinis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Phe | - |
Rhodotorula glutinis | (E)-cinnamate + NH3 | - |
? | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
