Literature summary for 4.3.1.24 extracted from

Hu, G.; Jia, J.; Hur, Y.; Chung, Y.; Lee, J.; Yun, D.; Chung, W.; Yi, G.; Kim, T.; Kim, D.
Molecular characterization of phenylalanine ammonia lyase gene from Cistanche deserticola (2010), Mol. Biol. Rep., 38, 3741-3750.

Search for more literature for 4.3.1.24

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Cistanche deserticola

Inhibitors

Inhibitors	Comment	Organism	Structure
2-aminoindan-2-phosphonic acid	competitive, specific inhibitor	Cistanche deserticola
chlorogenic acid	uncompetitive	Cistanche deserticola
Cinnamic acid	mixed type	Cistanche deserticola
cinnamyl alcohol	uncompetitive	Cistanche deserticola
coniferyl alcohol	competitive	Cistanche deserticola
coniferyl aldehyde	competitive	Cistanche deserticola
coumaric acid	uncompetitive	Cistanche deserticola
Hg2+	1 mM, 65% loss of activity	Cistanche deserticola
additional information	not inhibitory: tyrosol, caffeic acid	Cistanche deserticola
Pb2+	1 mM, 50% loss of activity	Cistanche deserticola
salidroside	competitive	Cistanche deserticola
Zn2+	1 mM, 65% loss of activity	Cistanche deserticola

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.101	-	L-Phe	pH 8.5, 55°C	Cistanche deserticola

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cs+	1 mM, weak enhancement	Cistanche deserticola
Mn2+	1 mM, weak enhancement	Cistanche deserticola

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
94000	-	x * 94000, SDS-PAGE of recombinant protein with GST-tag	Cistanche deserticola

Organism

Organism	UniProt	Comment	Textmining
Cistanche deserticola	D3Y5L6	isoform PAL1	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-Phe	-	Cistanche deserticola	(E)-cinnamate + NH3	-	?
additional information	no substrate: L-Tyr	Cistanche deserticola	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 94000, SDS-PAGE of recombinant protein with GST-tag	Cistanche deserticola

Synonyms

Synonyms	Comment	Organism
PAL1	-	Cistanche deserticola

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
55	-	-	Cistanche deserticola

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
70	-	20 min, retains more than 85% of activity	Cistanche deserticola
75	-	20 min, loss of more than 85% of activity	Cistanche deserticola

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.36	-	L-Phe	pH 8.5, 55°C	Cistanche deserticola

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	-	Cistanche deserticola

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.000056	-	2-aminoindan-2-phosphonic acid	pH 8.5, 55°C	Cistanche deserticola
0.008	-	Cinnamic acid	pH 8.5, 55°C	Cistanche deserticola
0.013	-	coniferyl aldehyde	pH 8.5, 55°C	Cistanche deserticola
0.017	-	salidroside	pH 8.5, 55°C	Cistanche deserticola
0.032	-	coniferyl alcohol	pH 8.5, 55°C	Cistanche deserticola
0.032	-	coumaric acid	pH 8.5, 55°C	Cistanche deserticola
0.037	-	cinnamyl alcohol	pH 8.5, 55°C	Cistanche deserticola
0.043	-	chlorogenic acid	pH 8.5, 55°C	Cistanche deserticola

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
33.17	-	L-Phe	pH 8.5, 55°C	Cistanche deserticola

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Release 2023.1 (January 2023)