Literature summary for 4.3.1.24 extracted from

Hsieh, L.S.; Hsieh, Y.L.; Yeh, C.S.; Cheng, C.Y.; Yang, C.C.; Lee, P.D.
Molecular characterization of a phenylalanine ammonia-lyase gene (BoPAL1) from Bambusa oldhamii (2010), Mol. Biol. Rep., 38, 283-290.

Search for more literature for 4.3.1.24

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Pichia pastoris strain X-33	Bambusa oldhamii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.01	-	L-phenylalanine	recombinant enzyme, in 50 mM Tris-HCl, pH 8.5, at 37°C	Bambusa oldhamii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
76950	-	subunit, calculated from amino acid sequence	Bambusa oldhamii
80000	-	4 * 80000, subunit, SDS-PAGE	Bambusa oldhamii
323000	-	gel filtration	Bambusa oldhamii

Organism

Organism	UniProt	Comment	Textmining
Bambusa oldhamii	Q6ST23	-	-

Purification (Commentary)

Purification (Comment)	Organism
HiTrap Ni2+-chelating column chromatography	Bambusa oldhamii

Source Tissue

Source Tissue	Comment	Organism	Textmining
shoot	-	Bambusa oldhamii	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-phenylalanine	-	Bambusa oldhamii	(E)-cinnamate + NH3	-	?

Subunits

Subunits	Comment	Organism
homotetramer	4 * 80000, subunit, SDS-PAGE	Bambusa oldhamii

Synonyms

Synonyms	Comment	Organism
EC 4.3.1.5	formerly	Bambusa oldhamii
PAL1	isoform	Bambusa oldhamii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Bambusa oldhamii

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
40	70	-	Bambusa oldhamii

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
10.11	-	L-phenylalanine	recombinant enzyme, in 50 mM Tris-HCl, pH 8.5, at 37°C	Bambusa oldhamii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	-	Bambusa oldhamii

pH Range

pH Minimum	pH Maximum	Comment	Organism
7.5	10.5	-	Bambusa oldhamii

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
10	-	L-phenylalanine	recombinant enzyme, in 50 mM Tris-HCl, pH 8.5, at 37°C	Bambusa oldhamii

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Release 2023.1 (January 2023)