Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.3.1.24 extracted from

  • Koukol, J.; Conn, E.E.
    The metabolism of aromatic compounds in higher plants. IV. Purification and properties of the phenylalanine deaminase of Hordeum vulgare (1961), J. Biol. Chem., 236, 2692-2698.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
3,4-dihydroxyphenyl-DL-Ala
-
Hordeum vulgare
4-coumarate trans-p-coumarate, competitive Hordeum vulgare
4-coumarate
-
Medicago sativa
4-coumarate
-
Oryza sativa
beta-phenyl-DL-Ser
-
Hordeum vulgare
Ca2+ CaCl2, 1 mM, 57% inhibition Hordeum vulgare
Cd2+ CdCl2, 1 mM, 100% inhibition Hordeum vulgare
Cinnamic acid trans-cinnamic acid Hordeum vulgare
Cinnamic acid
-
Medicago sativa
Cinnamic acid
-
Oryza sativa
CN- KCN, 1 mM, 85% inhibition Hordeum vulgare
Cu2+ CuSO4, 1 mM, 18% inactivation Hordeum vulgare
GSH
-
Hordeum vulgare
Hg2+ HgCl2, 0.01 mM, 100% inhibition Hordeum vulgare
iodoacetamide
-
Hordeum vulgare
L-Cys
-
Hordeum vulgare
p-chloromercuriphenylsulfonic acid
-
Hordeum vulgare
Tyr competitive; L-Tyr Hordeum vulgare
Tyr
-
Medicago sativa
Zn2+ ZnSO4, 1 mM, 32% inhibition Hordeum vulgare

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7
-
L-Phe
-
Hordeum vulgare

Organism

Organism UniProt Comment Textmining
Hordeum vulgare
-
L. var. Aravat
-
Lupinus sp.
-
-
-
Medicago sativa
-
-
-
Melilotus officinalis
-
-
-
Oryza sativa
-
-
-
Pisum sativum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Hordeum vulgare

Storage Stability

Storage Stability Organism
-10°C, 25% loss of activity after 3.5 months Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Phe
-
Lupinus sp. (E)-cinnamate + NH3
-
?
L-Phe
-
Pisum sativum (E)-cinnamate + NH3
-
?
L-Phe
-
Medicago sativa (E)-cinnamate + NH3
-
?
L-Phe
-
Oryza sativa (E)-cinnamate + NH3
-
?
L-Phe
-
Melilotus officinalis (E)-cinnamate + NH3
-
?
L-Phe no activity with D-Phe Hordeum vulgare (E)-cinnamate + NH3
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8 9.2
-
Hordeum vulgare
8.8 9.2
-
Medicago sativa

pH Range

pH Minimum pH Maximum Comment Organism
7 10.5 pH 7: about 35% of maximal activity, pH 10.5: about 70% of maximal activity Hordeum vulgare