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Literature summary for 4.3.1.2 extracted from

  • Raj, H.; Szymanski, W.; de Villiers, J.; Rozeboom, H.J.; Veetil, V.P.; Reis, C.R.; de Villiers, M.; Dekker, F.J.; de Wildeman, S.; Quax, W.J.; Thunnissen, A.M.; Feringa, B.L.; Janssen, D.B.; Poelarends, G.J.
    Engineering methylaspartate ammonia lyase for the asymmetric synthesis of unnatural amino acids (2012), Nat. Chem., 4, 478-484.
    View publication on PubMed
Search for more literature for 4.3.1.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Clostridium tetanomorphum

Protein Variants

Protein Variants Comment Organism
L384A mutant displays pronounced amination activity towards 2-hexylfumarate ad decreased amination activity towards mesaconate compared to wild-type. Mutant displays a broad electrophile scope including fumarate derivatives with alkyl, aryl, alkoxy, aryloxy, alkylthio and arylthio substituents at the C2 position Clostridium tetanomorphum
Q73A mutant displays significant decrease in ammonia addition and a large increase in methylamine addition compared to wild-type. Mutant displays a wide nucleophile scope including structurally diverse linear and cyclic allkylamines Clostridium tetanomorphum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Clostridium tetanomorphum

Organism

Organism UniProt Comment Textmining
Clostridium tetanomorphum Q05514
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E)-2-butylbut-2-enedioate + NH3 wild-type: conversion 0%, mutant L384A: conversion 59% Clostridium tetanomorphum ?
-
 r
(2E)-2-ethylbut-2-enedioate + NH3 wild-type: conversion 74%, mutant L384A: conversion 76% Clostridium tetanomorphum ?
-
 r
(2E)-2-hexylbut-2-enedioate + NH3 wild-type: conversion 0%, mutant L384A: conversion 53% Clostridium tetanomorphum ?
-
 r
(2E)-2-methylbut-2-enedioate + NH3 wild-type: conversion 80%, mutant L384A: conversion 75% Clostridium tetanomorphum ?
-
 r
(2E)-2-pentylbut-2-enedioate + NH3 wild-type: conversion 0%, mutant L384A: conversion 52% Clostridium tetanomorphum ?
-
 r
(2E)-2-phenylbut-2-enedioate + NH3 wild-type: conversion 0%, mutant L384A: conversion 90% Clostridium tetanomorphum ?
-
 r
(2E)-2-propylbut-2-enedioate + NH3 wild-type: conversion 57%, mutant L384A: conversion 67% Clostridium tetanomorphum ?
-
 r
(2Z)-2-(benzyloxy)but-2-enedioate + NH3 wild-type: conversion 0%, mutant L384A: conversion 60% Clostridium tetanomorphum ?
-
 r
(2Z)-2-(benzylsulfanyl)but-2-enedioate + NH3 wild-type: conversion 0%, mutant L384A: conversion 42% Clostridium tetanomorphum ?
-
 r
(2Z)-2-(ethylsulfanyl)but-2-enedioate + NH3 wild-type: conversion 36%, mutant L384A: conversion 50% Clostridium tetanomorphum ?
-
 r
(2Z)-2-(phenylsulfanyl)but-2-enedioate + NH3 wild-type: conversion 0%, mutant L384A: conversion 34% Clostridium tetanomorphum ?
-
 r
(2Z)-2-ethoxybut-2-enedioate + NH3 wild-type: conversion 46%, mutant L384A: conversion 43% Clostridium tetanomorphum ?
-
 r
(2Z)-2-phenoxybut-2-enedioate + NH3 wild-type: conversion 0%, mutant L384A: conversion 44% Clostridium tetanomorphum ?
-
 r
mesaconate + (aminooxy)methane mutant Q73A, conversion: 99%, excess of threo isomer over erythro isomer: more than 95% Clostridium tetanomorphum ?
-
 r
mesaconate + 1-cyclopropylmethanamine mutant Q73A, conversion: 61%, excess of threo isomer over erythro isomer: more than 95%, enantiomeric excess: more than 99% Clostridium tetanomorphum ?
-
 r
mesaconate + 2-aminoethanol mutant Q73A, conversion: 37%, excess of threo isomer over erythro isomer: more than 95% Clostridium tetanomorphum ?
-
 r
mesaconate + 2-methoxyethanamine mutant Q73A, conversion: 60%, excess of threo isomer over erythro isomer: more than 95% Clostridium tetanomorphum ?
-
 r
mesaconate + 3-aminopropan-1-ol mutant Q73A, conversion: 56%, excess of threo isomer over erythro isomer: 80% Clostridium tetanomorphum ?
-
 r
mesaconate + aminobutane mutant Q73A, conversion: 60%, excess of threo isomer over erythro isomer: more than 95%, enantiomeric excess: more than 99% Clostridium tetanomorphum ?
-
 r
mesaconate + aniline mutant Q73A, conversion: 6%, excess of threo isomer over erythro isomer: more than 95% Clostridium tetanomorphum ?
-
 r
mesaconate + cyclobutanamine mutant Q73A, conversion: 65%, excess of threo isomer over erythro isomer: more than 95%, enantiomeric excess: more than 99% Clostridium tetanomorphum ?
-
 r
mesaconate + cyclohexanamine mutant Q73A, conversion: 21%, excess of threo isomer over erythro isomer: more than 95% Clostridium tetanomorphum ?
-
 r
mesaconate + cyclopentanamine mutant Q73A, conversion: 36%, excess of threo isomer over erythro isomer: more than 95% Clostridium tetanomorphum ?
-
 r
mesaconate + cyclopropanamine mutant Q73A, conversion: 75%, excess of threo isomer over erythro isomer: more than 95% Clostridium tetanomorphum ?
-
 r
mesaconate + ethanamine mutant Q73A, conversion: 51%, excess of threo isomer over erythro isomer: more than 95%, enantiomeric excess: more than 99% Clostridium tetanomorphum ?
-
 r
mesaconate + ethane-1,2-diamine mutant Q73A, conversion: 90%, excess of threo isomer over erythro isomer: more than 95% Clostridium tetanomorphum ?
-
 r
mesaconate + hexanamine mutant Q73A, conversion: 17%, excess of threo isomer over erythro isomer: more than 95% Clostridium tetanomorphum ?
-
 r
mesaconate + methanamine mutant Q73A, conversion: 74%, excess of threo isomer over erythro isomer: more than 95% Clostridium tetanomorphum ?
-
 r
mesaconate + N-methylethane-1,2-diamine mutant Q73A, conversion: 80%, excess of threo isomer over erythro isomer: more than 95% Clostridium tetanomorphum ?
-
 r
mesaconate + NH3 mutant Q73A, conversion: 79%, excess of threo isomer over erythro isomer: 5% Clostridium tetanomorphum L-threo-3-methylaspartate
-
 r
mesaconate + NH3 wild-type: conversion 100%, mutant L384A: conversion 99% Clostridium tetanomorphum L-threo-3-methylaspartate
-
 r
mesaconate + pentanamine mutant Q73A, conversion: 29%, excess of threo isomer over erythro isomer: more than 95% Clostridium tetanomorphum ?
-
 r
mesaconate + propan-2-amine mutant Q73A, conversion: 8%, excess of threo isomer over erythro isomer: more than 95%, enantiomeric excess: more than 99% Clostridium tetanomorphum ?
-
 r
mesaconate + propanamine mutant Q73A, conversion: 53%, excess of threo isomer over erythro isomer: more than 95%, enantiomeric excess: more than 99% Clostridium tetanomorphum ?
-
 r
mesaconate + propane-1,3-diamine mutant Q73A, conversion: 78%, excess of threo isomer over erythro isomer: more than 95% Clostridium tetanomorphum ?
-
 r

Synonyms

Synonyms Comment Organism
MAL
-
 Clostridium tetanomorphum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at Clostridium tetanomorphum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 assay at Clostridium tetanomorphum