Literature summary for 4.3.1.2 extracted from

Levy, C.W.; Buckley, P.A.; Sedelnikova, S.; Kato, Y.; Asano, Y.; Rice, D.W.; Baker, P.J.
Insights into enzyme evolution revealed by the structure of methylaspartate ammonia lyase (2002), Structure, 10, 105-113.

Search for more literature for 4.3.1.2

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Citrobacter amalonaticus

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapour diffusion, well solution consists of 100 mM potassium phosphate, pH 8.0, and 32% ammonium sulfate, crystals diffract to 1.3 A	Citrobacter amalonaticus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
K+	required for activity	Citrobacter amalonaticus
Mg2+	required for actiivty	Citrobacter amalonaticus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
45500	-	2 * 45500	Citrobacter amalonaticus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-threo-3-methylaspartate	Citrobacter amalonaticus	-	mesaconate + NH3	-	r

Organism

Organism	UniProt	Comment	Textmining
Citrobacter amalonaticus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant 3-methylaspartase	Citrobacter amalonaticus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2S,3S)-3-ethylaspartate	-	Citrobacter amalonaticus	?	-	?
L-threo-3-methylaspartate	-	Citrobacter amalonaticus	mesaconate + NH3	-	r

Subunits

Subunits	Comment	Organism
dimer	2 * 45500	Citrobacter amalonaticus

Synonyms

Synonyms	Comment	Organism
MAL	-	Citrobacter amalonaticus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)