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Literature summary for 4.3.1.19 extracted from

  • Oda, Y.; Nakano, Y.; Kitaoka, S.
    Occurence and some properties of two threonine dehydratases in Euglena gracilis (1983), J. Gen. Microbiol., 129, 57-61.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
additional information dehydratase I and II: not affected by adenylates Euglena gracilis

General Stability

General Stability Organism
loss of activity of dehydratase I at 37 C is prevented by 1 mM Ile, but that of dehydratase II is not Euglena gracilis

Inhibitors

Inhibitors Comment Organism Structure
Ile dehydratase I, 50% inhibition at 0.35 mM Euglena gracilis
Leu dehydratase I, 6 mM, 50% inhibition Euglena gracilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
13
-
L-Thr dehydratase II Euglena gracilis
34
-
L-Thr dehydratase I Euglena gracilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
250000
-
dehydratase II, gel filtration Euglena gracilis

Organism

Organism UniProt Comment Textmining
Euglena gracilis
-
dehydratase I and dehydratase II
-
Euglena gracilis SM-ZK
-
dehydratase I and dehydratase II
-

Purification (Commentary)

Purification (Comment) Organism
partial Euglena gracilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Euglena gracilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine dehydratase I: 26% of the activity with L-Thr, dehydratase II: 16% of the activity with L-Thr Euglena gracilis pyruvate + NH3
-
?
L-serine dehydratase I: 26% of the activity with L-Thr, dehydratase II: 16% of the activity with L-Thr Euglena gracilis SM-ZK pyruvate + NH3
-
?
L-threonine
-
Euglena gracilis 2-oxobutanoate + NH3
-
?
L-threonine
-
Euglena gracilis SM-ZK 2-oxobutanoate + NH3
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
10 min, 16% loss of activity of dehydratase I and 36% loss of activity of dehydratase II, loss of activity of dehydratase I is prevented by 1 mM Ile, but that of dehydratase II is not Euglena gracilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
dehydratase II Euglena gracilis
9.5 11 dehydratase I Euglena gracilis