Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | dehydratase I and II: not affected by adenylates | Euglena gracilis |
General Stability | Organism |
---|---|
loss of activity of dehydratase I at 37 C is prevented by 1 mM Ile, but that of dehydratase II is not | Euglena gracilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ile | dehydratase I, 50% inhibition at 0.35 mM | Euglena gracilis | |
Leu | dehydratase I, 6 mM, 50% inhibition | Euglena gracilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13 | - |
L-Thr | dehydratase II | Euglena gracilis | |
34 | - |
L-Thr | dehydratase I | Euglena gracilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
250000 | - |
dehydratase II, gel filtration | Euglena gracilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Euglena gracilis | - |
dehydratase I and dehydratase II | - |
Euglena gracilis SM-ZK | - |
dehydratase I and dehydratase II | - |
Purification (Comment) | Organism |
---|---|
partial | Euglena gracilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Euglena gracilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine | dehydratase I: 26% of the activity with L-Thr, dehydratase II: 16% of the activity with L-Thr | Euglena gracilis | pyruvate + NH3 | - |
? | |
L-serine | dehydratase I: 26% of the activity with L-Thr, dehydratase II: 16% of the activity with L-Thr | Euglena gracilis SM-ZK | pyruvate + NH3 | - |
? | |
L-threonine | - |
Euglena gracilis | 2-oxobutanoate + NH3 | - |
? | |
L-threonine | - |
Euglena gracilis SM-ZK | 2-oxobutanoate + NH3 | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
10 min, 16% loss of activity of dehydratase I and 36% loss of activity of dehydratase II, loss of activity of dehydratase I is prevented by 1 mM Ile, but that of dehydratase II is not | Euglena gracilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
dehydratase II | Euglena gracilis |
9.5 | 11 | dehydratase I | Euglena gracilis |