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Literature summary for 4.3.1.17 extracted from
- Kinetic, mutagenic, and structural homology analysis of L-serine dehydratase from Legionella pneumophila (2011), Arch. Biochem. Biophys., 515, 28-36.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | development of a simple, direct, and continuous assay based on the absorbance of pyruvate, the product of the dehydratase reaction | Legionella pneumophila |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C183A | 135% of wild-type activity. Enzyme retains the positive cooperativity seen in the native enzyme | Legionella pneumophila |
| C221A | 70% of wild-type activity. Enzyme retains the positive cooperativity seen in the native enzyme | Legionella pneumophila |
| C226A | 100% of wild-type activity. Enzyme retains the positive cooperativity seen in the native enzyme | Legionella pneumophila |
| C304A | 10% of wild-type activity. Mutant displays altered kinetics with a higher Km and a Hill coefficient indicating negative homotropic cooperativity | Legionella pneumophila |
| C343A | inactive, mutants lacks the charge transfer absorbance at 400 nm that is indicative of the 4Fe-4S center | Legionella pneumophila |
| C385A | inactive, mutants lacks the charge transfer absorbance at 400 nm that is indicative of the 4Fe-4S center | Legionella pneumophila |
| C396A | inactive, mutants lacks the charge transfer absorbance at 400 nm that is indicative of the 4Fe-4S center | Legionella pneumophila |
| C458A | 44% of wild-type activity. Enzyme retains the positive cooperativity seen in the native enzyme | Legionella pneumophila |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ammonia | product inhibition, noncompetitive | Legionella pneumophila |  |
| D-serine | competitive | Legionella pneumophila | |
| L-cysteine | competitive | Legionella pneumophila | |
| pyruvate | product inhibition, competitive | Legionella pneumophila | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
L-Ser | wild-type, pH 7.0, temperature not specified in the publication | Legionella pneumophila | |
| 2.2 | - |
L-Ser | mutant C183A, pH 7.0, temperature not specified in the publication | Legionella pneumophila | |
| 2.7 | - |
L-Ser | mutant C226A, pH 7.0, temperature not specified in the publication | Legionella pneumophila | |
| 3 | - |
L-Ser | mutant C221A, pH 7.0, temperature not specified in the publication | Legionella pneumophila | |
| 6.9 | - |
L-Ser | mutant C458A, pH 7.0, temperature not specified in the publication | Legionella pneumophila | |
| 17.8 | - |
L-Ser | mutant C304A, pH 7.0, temperature not specified in the publication | Legionella pneumophila | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Legionella pneumophila | Q5ZXE1 | type 2 enzyme | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Ser | - |
Legionella pneumophila | pyruvate + NH3 | - |
ir | |
| L-Thr | Km -value is approximately 150 times that for L-serine. At an L-threonine concentration of 300 mM, the enzyme activity is approximately 5% of the activity for L-serine at its Km of 2 mM | Legionella pneumophila | 2-oxobutanoate + NH3 | - |
? | |
| additional information | enzyme shows homotopic cooperativity, Hill coefficient of wild-type is 1.7 | Legionella pneumophila | ? | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 323 | - |
L-Ser | mutant C304A, pH 7.0, temperature not specified in the publication | Legionella pneumophila | |
| 330 | - |
L-Ser | wild-type, pH 7.0, temperature not specified in the publication | Legionella pneumophila | |
| 359 | - |
L-Ser | mutant C221A, pH 7.0, temperature not specified in the publication | Legionella pneumophila | |
| 467 | - |
L-Ser | mutant C226A, pH 7.0, temperature not specified in the publication | Legionella pneumophila | |
| 506 | - |
L-Ser | mutant C183A, pH 7.0, temperature not specified in the publication | Legionella pneumophila | |
| 512 | - |
L-Ser | mutant C458A, pH 7.0, temperature not specified in the publication | Legionella pneumophila | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | braod | Legionella pneumophila |
| 7 | - |
assay at | Legionella pneumophila |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
L-cysteine | pH 7.0, temperature not specified in the publication | Legionella pneumophila | |
| 10 | - |
D-serine | pH 7.0, temperature not specified in the publication | Legionella pneumophila | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 18 | - |
L-Ser | mutant C304A, pH 7.0, temperature not specified in the publication | Legionella pneumophila | |
| 74 | - |
L-Ser | mutant C458A, pH 7.0, temperature not specified in the publication | Legionella pneumophila | |
| 120 | - |
L-Ser | mutant C221A, pH 7.0, temperature not specified in the publication | Legionella pneumophila | |
| 170 | - |
L-Ser | mutant C226A, pH 7.0, temperature not specified in the publication | Legionella pneumophila | |
| 170 | - |
L-Ser | wild-type, pH 7.0, temperature not specified in the publication | Legionella pneumophila | |
| 230 | - |
L-Ser | mutant C183A, pH 7.0, temperature not specified in the publication | Legionella pneumophila | |
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