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Literature summary for 4.3.1.17 extracted from

  • Burman, J.D.; Harris, R.L.; Hauton, K.A.; Lawson, D.M.; Sawers, R.G.
    The iron-sulfur cluster in the L-serine dehydratase TdcG from Escherichia coli is required for enzyme activity (2004), FEBS Lett., 576, 442-444.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.8
-
L-serine anaerobically isolated enzyme, pH 8.0 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Iron 7.7 mol iron per mol dimer, two (4Fe-4S)2+ clusters per dimer in anaerobically isolated enzyme, exposure to air results in loss of clusters and concomitant loss of enzyme activity Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
2 * 52910, calculated for His-tagged protein, 2 * 48460, calculated for native protein, 2 * 46000, SDS-PAGE of His-tagged protein Escherichia coli
48460
-
2 * 52910, calculated for His-tagged protein, 2 * 48460, calculated for native protein, 2 * 46000, SDS-PAGE of His-tagged protein Escherichia coli
52910
-
2 * 52910, calculated for His-tagged protein, 2 * 48460, calculated for native protein, 2 * 46000, SDS-PAGE of His-tagged protein Escherichia coli
101700
-
dynamic light scattering Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
anaerobically inducible enzyme
-

Purification (Commentary)

Purification (Comment) Organism
anaerobic purification of His-tagged enzyme, purified protein is brown in colour Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
307
-
anaerobically isolated enzyme, pH 8.0 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine
-
Escherichia coli pyruvate + NH3
-
?
additional information no substrate: L-threonine Escherichia coli ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 52910, calculated for His-tagged protein, 2 * 48460, calculated for native protein, 2 * 46000, SDS-PAGE of His-tagged protein Escherichia coli

Synonyms

Synonyms Comment Organism
TdcG
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
544
-
L-serine anaerobically isolated enzyme, pH 8.0 Escherichia coli