KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.8 | - |
L-serine | anaerobically isolated enzyme, pH 8.0 | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | 7.7 mol iron per mol dimer, two (4Fe-4S)2+ clusters per dimer in anaerobically isolated enzyme, exposure to air results in loss of clusters and concomitant loss of enzyme activity | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
2 * 52910, calculated for His-tagged protein, 2 * 48460, calculated for native protein, 2 * 46000, SDS-PAGE of His-tagged protein | Escherichia coli |
48460 | - |
2 * 52910, calculated for His-tagged protein, 2 * 48460, calculated for native protein, 2 * 46000, SDS-PAGE of His-tagged protein | Escherichia coli |
52910 | - |
2 * 52910, calculated for His-tagged protein, 2 * 48460, calculated for native protein, 2 * 46000, SDS-PAGE of His-tagged protein | Escherichia coli |
101700 | - |
dynamic light scattering | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
anaerobically inducible enzyme | - |
Purification (Comment) | Organism |
---|---|
anaerobic purification of His-tagged enzyme, purified protein is brown in colour | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
307 | - |
anaerobically isolated enzyme, pH 8.0 | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine | - |
Escherichia coli | pyruvate + NH3 | - |
? | |
additional information | no substrate: L-threonine | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 52910, calculated for His-tagged protein, 2 * 48460, calculated for native protein, 2 * 46000, SDS-PAGE of His-tagged protein | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
TdcG | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
544 | - |
L-serine | anaerobically isolated enzyme, pH 8.0 | Escherichia coli |