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Literature summary for 4.3.1.17 extracted from

  • Velayudhan, J.; Jones, M.A.; Barrow, P.A.; Kelly, D.J.
    L-serine catabolism via an oxygen-labile L-serine dehydratase is essential for colonization of the avian gut by Campylobacter jejuni (2004), Infect. Immun., 72, 260-268.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Campylobacter jejuni

Inhibitors

Inhibitors Comment Organism Structure
D-serine 100 mM, 40% inhibition Campylobacter jejuni
L-cysteine 50 mM, 95% inhibition Campylobacter jejuni
L-threonine 100 mM, 56% inhibition Campylobacter jejuni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
14.6
-
L-serine
-
Campylobacter jejuni

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ iron-sulfur enzyme Campylobacter jejuni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51758
-
2 * 51758, electrospray mass spectrometry Campylobacter jejuni
123000
-
gel filtration Campylobacter jejuni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-serine Campylobacter jejuni
-
pyruvate + NH3
-
?

Organism

Organism UniProt Comment Textmining
Campylobacter jejuni
-
-
-

Oxidation Stability

Oxidation Stability Organism
purified SdaA is inactivated with a half-life of approx. 1.5 h upon exposure to air, inactivated SdaA can be reactivated to approx. 60% of its activity under strict anaerobic conditiones with Fe2+ and dithiothreitol Campylobacter jejuni

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged SdaA Campylobacter jejuni

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
115
-
recombinant SdaA Campylobacter jejuni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine
-
Campylobacter jejuni pyruvate + NH3
-
?

Subunits

Subunits Comment Organism
dimer 2 * 51758, electrospray mass spectrometry Campylobacter jejuni

Cofactor

Cofactor Comment Organism Structure
additional information pyridoxal 5'-phosphate independent Campylobacter jejuni