Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Campylobacter jejuni |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-serine | 100 mM, 40% inhibition | Campylobacter jejuni | |
L-cysteine | 50 mM, 95% inhibition | Campylobacter jejuni | |
L-threonine | 100 mM, 56% inhibition | Campylobacter jejuni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14.6 | - |
L-serine | - |
Campylobacter jejuni |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | iron-sulfur enzyme | Campylobacter jejuni |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51758 | - |
2 * 51758, electrospray mass spectrometry | Campylobacter jejuni |
123000 | - |
gel filtration | Campylobacter jejuni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine | Campylobacter jejuni | - |
pyruvate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Campylobacter jejuni | - |
- |
- |
Oxidation Stability | Organism |
---|---|
purified SdaA is inactivated with a half-life of approx. 1.5 h upon exposure to air, inactivated SdaA can be reactivated to approx. 60% of its activity under strict anaerobic conditiones with Fe2+ and dithiothreitol | Campylobacter jejuni |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged SdaA | Campylobacter jejuni |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
115 | - |
recombinant SdaA | Campylobacter jejuni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine | - |
Campylobacter jejuni | pyruvate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 51758, electrospray mass spectrometry | Campylobacter jejuni |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | pyridoxal 5'-phosphate independent | Campylobacter jejuni |