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Literature summary for 4.3.1.17 extracted from

  • Grabowski, R.; Buckel, W.
    Purification and properties of an iron-sulfur containing and pyridoxal-phosphate-independent L-serine dehydratase from Peptostreptococcus asaccharolyticus (1991), Eur. J. Biochem., 199, 89-94.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline no effect Peptoniphilus asaccharolyticus
EDTA no effect Peptoniphilus asaccharolyticus
ethanolamine
-
Peptoniphilus asaccharolyticus
Gly
-
Peptoniphilus asaccharolyticus
glycylglycine
-
Peptoniphilus asaccharolyticus
L-Cys competitive Peptoniphilus asaccharolyticus
PCMB
-
Peptoniphilus asaccharolyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
L-Ser
-
Peptoniphilus asaccharolyticus

Metals/Ions

Metals/Ions Comment Organism Structure
Iron the enzyme contains 3.8 mol Fe and 5.6 mol inorganic sulfur per mol of heterodimer, indicating the presence of an [Fe-S]center Peptoniphilus asaccharolyticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
180000
-
gradient PAGE Peptoniphilus asaccharolyticus
200000
-
gel filtration Peptoniphilus asaccharolyticus

Organism

Organism UniProt Comment Textmining
Peptoniphilus asaccharolyticus
-
-
-

Oxidation Stability

Oxidation Stability Organism
rapid loss of activity upon exposure to air, reactivation by Fe2+ Peptoniphilus asaccharolyticus

Purification (Commentary)

Purification (Comment) Organism
-
Peptoniphilus asaccharolyticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Peptoniphilus asaccharolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine specific for L-Ser Peptoniphilus asaccharolyticus pyruvate + NH3
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4
-
-
Peptoniphilus asaccharolyticus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate pyridoxal-5'-phosphate-independent enzyme Peptoniphilus asaccharolyticus