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Literature summary for 4.3.1.17 extracted from

  • Newman, E.B.; Kapoor, V.
    In vitro studies on L-serine deaminase activity of Escherichia coli K12 (1980), Can. J. Biochem., 58, 1292-1297.
    View publication on PubMed

General Stability

General Stability Organism
unstable in all attempts at purification Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Escherichia coli
AlK(SO4)2
-
Escherichia coli
CuCl2 0.7 mM, 50% inhibition Escherichia coli
D-Ser
-
Escherichia coli
ethanolamine
-
Escherichia coli
ZnSO4
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
420
-
L-Ser
-
Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
K12
-

Storage Stability

Storage Stability Organism
-10°C, 40% glycerol, loss of activity within 1 week Escherichia coli
0°C, 45% loss of activity after 30 min Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine
-
Escherichia coli pyruvate + NH3
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
-
30 min, 45% loss of activity Escherichia coli
37
-
complete loss of activity within 30 min. Fe2+, L-Ser D-Ser or ethanolamine decrease the loss of activity at 37°C Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.7
-
CuCl2
-
Escherichia coli