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Literature summary for 4.3.1.15 extracted from
- Cloning, expression, and sequence analysis of diaminopropionate ammonia lyase gene from a nonvirulent Salmonella typhimurium PU011 (2007), Appl. Biochem. Biotechnol., 141, 161-174.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | nonvirulent Salmonella typhimurium PU011 can be used for the production of the enzyme to detoxify the neurotoxin 3-oxalyl and 2,3-dioxalyl diaminopropionate | Salmonella enterica subsp. enterica serovar Typhimurium |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli PU018 | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.74 | - |
2,3-Diaminopropionate | wild type enzyme, 0.680 using Lathyrus sativus seed extracts as substrate | Salmonella enterica subsp. enterica serovar Typhimurium |  |
| 0.741 | - |
2,3-Diaminopropionate | recombinant enzyme, 0.683 using Lathyrus sativus seed extracts as substrate | Salmonella enterica subsp. enterica serovar Typhimurium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
SDS-PAGE of wild type and recombinant protein | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
PU011 | - |
| Salmonella enterica subsp. enterica serovar Typhimurium PU011 | - |
PU011 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.346 | - |
of the recombinant enzyme in crude extracts | Salmonella enterica subsp. enterica serovar Typhimurium |
| 0.351 | - |
of the wild type enzyme in crude extracts | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,3-diaminopropanoate + H2O | precursor of a neurotoxin in Lathyrus sativus seed extracts | Salmonella enterica subsp. enterica serovar Typhimurium | pyruvate + NH3 | - |
? | |
| 2,3-diaminopropanoate + H2O | precursor of a neurotoxin in Lathyrus sativus seed extracts | Salmonella enterica subsp. enterica serovar Typhimurium PU011 | pyruvate + NH3 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DAPAL | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
| diaminopropionate ammonia-lyase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
45 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
45 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
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