BRENDA - Enzyme Database show
show all sequences of 4.3.1.12

Crystallization and X-ray diffraction analysis of ornithine cyclodeaminase from Pseudomonas putida

Alam, S.; Wang, S.C.; Ruzicka, F.J.; Frey, P.A.; Wedekind, J.E.; Acta Crystallogr. Sect. D 60, 941-944 (2004)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli XL-2-Blue
Pseudomonas putida
Crystallization (Commentary)
Crystallization
Organism
vapor-diffusion method, the best diffraction-quality crystals are obtained from solutions of 40%(v/v) 2-methyl-2,4-pentanediol buffered at pH 6.0 with 0.1 M MES and diffracted X-rays to 1.68 A resolution. Crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 70.0, b = 78.3, c = 119.4 A
Pseudomonas putida
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
-
-
-
Purification (Commentary)
Commentary
Organism
-
Pseudomonas putida
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli XL-2-Blue
Pseudomonas putida
Crystallization (Commentary) (protein specific)
Crystallization
Organism
vapor-diffusion method, the best diffraction-quality crystals are obtained from solutions of 40%(v/v) 2-methyl-2,4-pentanediol buffered at pH 6.0 with 0.1 M MES and diffracted X-rays to 1.68 A resolution. Crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 70.0, b = 78.3, c = 119.4 A
Pseudomonas putida
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas putida
Other publictions for EC 4.3.1.12
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
729505
Sharma
Functional characterization of ...
Arabidopsis thaliana
BMC Plant Biol.
13
182
2013
-
-
-
-
-
-
-
-
1
-
-
-
-
5
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
2
2
1
-
-
730331
Jensen
Ornithine cyclodeaminase-based ...
Pseudomonas putida
Microb. Cell Fact.
12
63
2013
-
1
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
729853
Ion
A molecular dynamics (MD) and ...
Pseudomonas putida
Int. J. Mol. Sci.
13
12994-13011
2012
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
705095
Lee
Enhancement of ornithine produ ...
Corynebacterium glutamicum
J. Microbiol. Biotechnol.
20
127-131
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
704292
Fonknechten
A conserved gene cluster rules ...
Acetoanaerobium sticklandii
J. Bacteriol.
191
3162-3167
2009
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
693873
Cuervo
Differential soluble protein e ...
Trichomonas vaginalis
J. Proteomics
71
109-122
2008
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
695039
Ozyurt
Computational active site anal ...
Saccharolobus solfataricus
Proteins
72
184-196
2008
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
660605
Alam
Crystallization and X-ray diff ...
Pseudomonas putida
Acta Crystallogr. Sect. D
60
941-944
2004
-
-
1
1
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
661097
Goodman
Ornithine cyclodeaminase: stru ...
Pseudomonas putida
Biochemistry
43
13883-13891
2004
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
34272
Soto
-
Ornithine cyclodeaminase activ ...
Agrobacterium tumefaciens, Rhodobacter sp., Sinorhizobium meliloti, Sinorhizobium meliloti 2011, Sinorhizobium meliloti 41, Sinorhizobium meliloti GR4
FEMS Microbiol. Lett.
11
209-214
1994
1
-
-
-
-
-
-
1
-
-
-
11
-
7
-
-
-
-
-
-
2
1
17
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
2
-
-
-
-
-
-
1
-
-
-
11
-
-
-
-
-
-
2
1
17
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
34273
Soto
Identification of a novel Rhiz ...
Agrobacterium tumefaciens, Sinorhizobium meliloti, Sinorhizobium meliloti GR4
Mol. Plant Microbe Interact.
7
703-707
1994
-
-
-
-
-
-
-
-
-
-
-
6
-
10
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
34271
Schindler
Ornithine cyclodeaminase from ...
Agrobacterium tumefaciens
J. Bacteriol.
171
847-854
1989
-
-
1
-
-
-
-
3
-
-
2
1
-
5
-
-
-
-
-
-
1
1
2
1
3
-
-
-
2
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
3
-
-
2
1
-
-
-
-
-
-
1
1
2
1
3
-
-
-
2
-
-
-
-
-
-
-
-
-
34269
Sans
Ornithine cyclodeaminase from ...
Agrobacterium tumefaciens
Eur. J. Biochem.
173
123-130
1988
2
-
1
-
-
-
-
3
-
-
-
1
-
6
-
-
-
-
-
-
-
-
2
-
2
-
-
-
2
-
-
1
-
-
-
2
-
1
1
-
-
-
-
-
-
3
-
-
-
1
-
-
-
-
-
-
-
-
2
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
34268
Stalon
Catabolism of arginine, citrul ...
Acetoanaerobium sticklandii, Burkholderia cepacia, Pseudomonas putida
J. Gen. Microbiol.
133
2487-2495
1987
-
-
-
-
-
-
-
-
-
-
-
3
-
7
-
-
-
-
-
-
2
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
2
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
34270
Sans
The Noc region of Ti plasmid C ...
Agrobacterium tumefaciens
Eur. J. Biochem.
167
81-87
1987
-
-
1
-
-
-
-
-
-
-
-
1
-
5
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
34267
Dessaux
Arginine catabolism in Agrobac ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens GV4R10c21, Agrobacterium tumefaciens R10, Clostridium sporogenes, Treponema denticola
J. Bacteriol.
166
44-50
1986
-
-
-
-
-
-
1
1
-
-
-
5
-
7
-
-
-
-
-
-
-
-
10
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
5
-
-
-
-
-
-
-
-
10
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
34265
Muth
Ornithine cyclase (deaminating ...
Clostridium sporogenes
J. Biol. Chem.
249
7457-7462
1974
-
-
-
-
-
1
4
2
-
-
3
1
-
1
2
-
1
-
-
-
2
2
2
1
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
1
-
4
-
2
-
-
3
1
-
2
-
1
-
-
2
2
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
34266
Muth
Ornithine cyclase (deaminating ...
Clostridium botulinum, Clostridium sporogenes
J. Biol. Chem.
249
7463-7467
1974
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
34264
Costilow
Ornithine cyclase (deaminating ...
Clostridium sporogenes
J. Biol. Chem.
246
6655-6660
1971
-
-
-
-
-
1
-
3
-
-
-
1
-
1
1
-
1
-
-
-
2
1
3
-
1
1
-
-
1
1
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
3
-
-
-
1
-
1
-
1
-
-
2
1
3
-
1
1
-
-
1
1
-
-
-
-
-
-
-
-