BRENDA - Enzyme Database show
show all sequences of 4.3.1.12

Ornithine cyclase (deaminating). Purification of a protein that converts ornithine to proline and definition of the optimal assay conditions

Costilow, R.N.; Laycock, L.; J. Biol. Chem. 246, 6655-6660 (1971)

Data extracted from this reference:

General Stability
General Stability
Organism
low concentrations of ornithine stabilize
Clostridium sporogenes
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0061
-
NAD+
-
Clostridium sporogenes
3.3
-
NADP+
-
Clostridium sporogenes
11.1
-
L-ornithine
-
Clostridium sporogenes
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-ornithine
Clostridium sporogenes
-
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Clostridium sporogenes
-
ATCC 7955; National Canners Association, PA 3679
-
Oxidation Stability
Oxidation Stability
Organism
enzyme activity in the presence of air is 80 to 90% of those in argon atmosphere
Clostridium sporogenes
Purification (Commentary)
Commentary
Organism
partial
Clostridium sporogenes
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Clostridium sporogenes
3.12
-
-
Clostridium sporogenes
Storage Stability
Storage Stability
Organism
-10°C, rapid loss of activity during storage
Clostridium sporogenes
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-ornithine
reaction is either irreversible or equilibrium is far in the direction of proline
34264
Clostridium sporogenes
L-proline + NH3
-
34264
Clostridium sporogenes
?
L-ornithine
specific
34264
Clostridium sporogenes
L-proline + NH3
-
34264
Clostridium sporogenes
?
L-ornithine
-
34264
Clostridium sporogenes
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
44
-
-
Clostridium sporogenes
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
33
52
50% of maximal activity at 33°C and 52°C
Clostridium sporogenes
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
8.2
-
Clostridium sporogenes
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.6
9.8
50% of maximal activity at pH 6.6 and pH 9.8
Clostridium sporogenes
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
required for maximal activity
Clostridium sporogenes
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
required for maximal activity
Clostridium sporogenes
General Stability (protein specific)
General Stability
Organism
low concentrations of ornithine stabilize
Clostridium sporogenes
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0061
-
NAD+
-
Clostridium sporogenes
3.3
-
NADP+
-
Clostridium sporogenes
11.1
-
L-ornithine
-
Clostridium sporogenes
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-ornithine
Clostridium sporogenes
-
?
-
-
-
Oxidation Stability (protein specific)
Oxidation Stability
Organism
enzyme activity in the presence of air is 80 to 90% of those in argon atmosphere
Clostridium sporogenes
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Clostridium sporogenes
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Clostridium sporogenes
3.12
-
-
Clostridium sporogenes
Storage Stability (protein specific)
Storage Stability
Organism
-10°C, rapid loss of activity during storage
Clostridium sporogenes
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-ornithine
reaction is either irreversible or equilibrium is far in the direction of proline
34264
Clostridium sporogenes
L-proline + NH3
-
34264
Clostridium sporogenes
?
L-ornithine
specific
34264
Clostridium sporogenes
L-proline + NH3
-
34264
Clostridium sporogenes
?
L-ornithine
-
34264
Clostridium sporogenes
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
44
-
-
Clostridium sporogenes
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
33
52
50% of maximal activity at 33°C and 52°C
Clostridium sporogenes
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
8.2
-
Clostridium sporogenes
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.6
9.8
50% of maximal activity at pH 6.6 and pH 9.8
Clostridium sporogenes
Other publictions for EC 4.3.1.12
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
729505
Sharma
Functional characterization of ...
Arabidopsis thaliana
BMC Plant Biol.
13
182
2013
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1
2
2
1
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730331
Jensen
Ornithine cyclodeaminase-based ...
Pseudomonas putida
Microb. Cell Fact.
12
63
2013
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1
1
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3
-
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1
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729853
Ion
A molecular dynamics (MD) and ...
Pseudomonas putida
Int. J. Mol. Sci.
13
12994-13011
2012
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705095
Lee
Enhancement of ornithine produ ...
Corynebacterium glutamicum
J. Microbiol. Biotechnol.
20
127-131
2010
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2
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704292
Fonknechten
A conserved gene cluster rules ...
Acetoanaerobium sticklandii
J. Bacteriol.
191
3162-3167
2009
-
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-
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-
-
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1
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1
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1
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1
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1
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1
1
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693873
Cuervo
Differential soluble protein e ...
Trichomonas vaginalis
J. Proteomics
71
109-122
2008
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1
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2
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1
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1
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1
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1
-
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-
695039
Ozyurt
Computational active site anal ...
Saccharolobus solfataricus
Proteins
72
184-196
2008
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1
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1
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1
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660605
Alam
Crystallization and X-ray diff ...
Pseudomonas putida
Acta Crystallogr. Sect. D
60
941-944
2004
-
-
1
1
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-
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2
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1
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1
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1
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-
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661097
Goodman
Ornithine cyclodeaminase: stru ...
Pseudomonas putida
Biochemistry
43
13883-13891
2004
-
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1
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-
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1
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1
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-
34272
Soto
-
Ornithine cyclodeaminase activ ...
Agrobacterium tumefaciens, Rhodobacter sp., Sinorhizobium meliloti, Sinorhizobium meliloti 2011, Sinorhizobium meliloti 41, Sinorhizobium meliloti GR4
FEMS Microbiol. Lett.
11
209-214
1994
1
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1
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11
-
7
-
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2
1
17
-
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2
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1
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2
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1
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11
-
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2
1
17
-
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34273
Soto
Identification of a novel Rhiz ...
Agrobacterium tumefaciens, Sinorhizobium meliloti, Sinorhizobium meliloti GR4
Mol. Plant Microbe Interact.
7
703-707
1994
-
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6
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10
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9
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6
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9
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34271
Schindler
Ornithine cyclodeaminase from ...
Agrobacterium tumefaciens
J. Bacteriol.
171
847-854
1989
-
-
1
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3
-
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2
1
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5
-
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1
1
2
1
3
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2
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1
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1
1
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3
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2
1
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1
1
2
1
3
-
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2
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-
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-
34269
Sans
Ornithine cyclodeaminase from ...
Agrobacterium tumefaciens
Eur. J. Biochem.
173
123-130
1988
2
-
1
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-
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3
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1
-
6
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2
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2
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2
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1
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1
1
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3
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1
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2
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2
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2
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34268
Stalon
Catabolism of arginine, citrul ...
Acetoanaerobium sticklandii, Burkholderia cepacia, Pseudomonas putida
J. Gen. Microbiol.
133
2487-2495
1987
-
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3
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7
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34270
Sans
The Noc region of Ti plasmid C ...
Agrobacterium tumefaciens
Eur. J. Biochem.
167
81-87
1987
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1
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34267
Dessaux
Arginine catabolism in Agrobac ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens GV4R10c21, Agrobacterium tumefaciens R10, Clostridium sporogenes, Treponema denticola
J. Bacteriol.
166
44-50
1986
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1
1
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5
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7
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10
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1
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1
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5
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10
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1
1
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34265
Muth
Ornithine cyclase (deaminating ...
Clostridium sporogenes
J. Biol. Chem.
249
7457-7462
1974
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1
4
2
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3
1
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1
2
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1
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2
2
2
1
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3
-
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3
-
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1
-
4
-
2
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3
1
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2
-
1
-
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2
2
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
34266
Muth
Ornithine cyclase (deaminating ...
Clostridium botulinum, Clostridium sporogenes
J. Biol. Chem.
249
7463-7467
1974
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-
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-
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2
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2
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1
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4
-
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1
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1
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2
-
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4
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
34264
Costilow
Ornithine cyclase (deaminating ...
Clostridium sporogenes
J. Biol. Chem.
246
6655-6660
1971
-
-
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-
1
-
3
-
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1
-
1
1
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1
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2
1
3
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1
1
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1
1
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1
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1
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1
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3
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1
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1
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1
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1
1
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1
1
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