Literature summary for 4.3.1.1 extracted from

Sheng, Y.; Li, S.; Gou, X.; Kong, X.; Wang, X.; Sun, Y.; Zhang, J.
The hybrid enzymes from alpha-aspartyl dipeptidase and L-aspartase (2005), Biochem. Biophys. Res. Commun., 331, 107-112.

Search for more literature for 4.3.1.1

Application

Application	Comment	Organism
synthesis	construction of hybrid enzyme from alpha-aspartyl dipeptidase and L-aspartase. The hybrid enzyme can be used in synthesis of the precursor for aspartame. Synthesizing aspartate from fumarate and NH4+, and then taking advantage of the catalytic action of alpha-aspartyl dipeptidase, the precursor of aspartame is produced	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of hybrid enzyme from alpha-aspartyl dipeptidase and L-aspartase. The hybrid enzyme can be used in synthesis of the precursor for aspartame	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	-	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-aspartate	-	Escherichia coli	fumarate + NH3	-	r

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	additional information	-	Escherichia coli

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)