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Literature summary for 4.3.1.1 extracted from

  • Kawata, Y.; Tamura, K.; Yano, S.; Mizobata, T.; Nagai, J.; Esaki, N.; Soda, K.; Tokushige, M.; Yumoto, N.
    Purification and characterization of thermostable aspartase from Bacillus sp. YM55-1 (1999), Arch. Biochem. Biophys., 366, 40-46.
    View publication on PubMed
Search for more literature for 4.3.1.1

General Stability

General Stability Organism
1 M guanidine hydrochloride, 80% residual activity Bacillus sp. (in: Bacteria)
after incubation in the presence of guanidine hydrochloride at a concentration of 1.1 M 50% loss of activity after 60 min Bacillus sp. (in: Bacteria)
after incubation in the presence of guanidine hydrochloride at a concentration of 1.1 M complete inactivation Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3
-
 L-aspartate 30°C Pseudomonas fluorescens
2.8
-
 L-aspartate 30°C Escherichia coli
28.5
-
 L-aspartate 30°C Bacillus sp. (in: Bacteria)
28.5
-
 L-aspartate 30°C, pH 8.5 Bacillus sp. (in: Bacteria)
32
-
 L-aspartate 55°C, pH 7.8 Bacillus sp. (in: Bacteria)
32
-
 L-aspartate 55°C Bacillus sp. (in: Bacteria)

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no activation by Mg2+ at alkaline pH Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
 4 * 51000, SDS-PAGE Bacillus sp. (in: Bacteria)
200000
-
 gel filtration Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli
-
 ?
-
 ?
additional information Pseudomonas fluorescens
-
 ?
-
 ?
additional information Bacillus sp. (in: Bacteria) important role in the bacterial nitrogen metabolism ?
-
 ?
additional information Bacillus sp. (in: Bacteria) YM55-1 important role in the bacterial nitrogen metabolism ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria)
-
 YM55-1
-
Bacillus sp. (in: Bacteria) YM55-1
-
-
-
Bacillus sp. (in: Bacteria) YM55-1
-
 YM55-1
-
Escherichia coli
-
-
-
Pseudomonas fluorescens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Escherichia coli
additional information
-
-
 Bacillus sp. (in: Bacteria)
167
-
 30°C Escherichia coli
214
-
 30°C Pseudomonas fluorescens
700
-
 30°C Bacillus sp. (in: Bacteria)
800
-
 55C, pH 8.0 Escherichia coli
2200
-
 55C Bacillus sp. (in: Bacteria)
2500
-
 65C, pH 7.5 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate
-
 Bacillus sp. (in: Bacteria) fumarate + NH3
-
 ?
L-aspartate r Escherichia coli fumarate + NH3
-
 ?
L-aspartate r Pseudomonas fluorescens fumarate + NH3
-
 ?
L-aspartate r Bacillus sp. (in: Bacteria) fumarate + NH3
-
 ?
L-aspartate natural substrate Escherichia coli fumarate + NH3
-
 ?
L-aspartate natural substrate Pseudomonas fluorescens fumarate + NH3
-
 ?
L-aspartate natural substrate Bacillus sp. (in: Bacteria) fumarate + NH3
-
 ?
L-aspartate
-
 Bacillus sp. (in: Bacteria) YM55-1 fumarate + NH3
-
 ?
L-aspartate r Bacillus sp. (in: Bacteria) YM55-1 fumarate + NH3
-
 ?
L-aspartate natural substrate Bacillus sp. (in: Bacteria) YM55-1 fumarate + NH3
-
 ?
additional information
-
 Escherichia coli ?
-
 ?
additional information
-
 Pseudomonas fluorescens ?
-
 ?
additional information
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
additional information important role in the bacterial nitrogen metabolism Bacillus sp. (in: Bacteria) ?
-
 ?
additional information
-
 Bacillus sp. (in: Bacteria) YM55-1 ?
-
 ?
additional information important role in the bacterial nitrogen metabolism Bacillus sp. (in: Bacteria) YM55-1 ?
-
 ?

Subunits

Subunits Comment Organism
tetramer
-
 Escherichia coli
tetramer
-
 Pseudomonas fluorescens
tetramer 4 * 51000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Escherichia coli
30
-
 assay at Bacillus sp. (in: Bacteria)
55
-
 assay at Bacillus sp. (in: Bacteria)
55
-
 at pH 8.0 Escherichia coli
65
-
 at pH 7.5 Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
-
 Escherichia coli
additional information
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
-
 Escherichia coli
additional information
-
 moderate thermophile Bacillus sp. (in: Bacteria)
55
-
 20% loss of activity after 60 min Bacillus sp. (in: Bacteria)
55
-
 complete loss of activity after 60 min Escherichia coli
55
-
 60 min, 80% residual activity Bacillus sp. (in: Bacteria)
75
-
 5 min, stable Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
-
 Escherichia coli
additional information
-
-
 Bacillus sp. (in: Bacteria)
7.5
-
-
 Escherichia coli
7.5
-
 assay at 60C Bacillus sp. (in: Bacteria)
8.5
-
-
 Escherichia coli
8.5
-
 assay at 30C Bacillus sp. (in: Bacteria)

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
-
 Escherichia coli
additional information
-
-
 Bacillus sp. (in: Bacteria)