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Literature summary for 4.3.1.1 extracted from
- Properties of alanine dehydrogenase and aspartase from Propionibacterium freudenreichii subsp. shermanii (1987), Appl. Environ. Microbiol., 53, 1885-1892.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | - |
Propionibacterium freudenreichii subsp. shermanii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| fumarate | 50% inhibition at a concentration of 0.2 to 0.6 mM, pH 6.2, 50% inhibition at a concentration of 1.1 to 2.5 mM, pH 7.5 | Propionibacterium freudenreichii subsp. shermanii |  |
| KCl | - |
Propionibacterium freudenreichii subsp. shermanii | |
| MgCl2 | inhibitor at high concentrations (above 10 mM) | Propionibacterium freudenreichii subsp. shermanii | |
| additional information | - |
Propionibacterium freudenreichii subsp. shermanii | |
| Na+ | - |
Propionibacterium freudenreichii subsp. shermanii | |
| pyruvate | - |
Propionibacterium freudenreichii subsp. shermanii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9.1 | - |
L-aspartate | 100 mM Bis-Tris-propane hydrochloride buffer, pH 6.2 | Propionibacterium freudenreichii subsp. shermanii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 190000 | - |
gel filtration | Propionibacterium freudenreichii subsp. shermanii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Propionibacterium freudenreichii subsp. shermanii | key enzyme involved in the metabolism of aspartate | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella aerogenes | - |
- |
- |
| Propionibacterium freudenreichii subsp. shermanii | - |
NCDO 566, ATCC 9614, MNS, DRI, KFA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Propionibacterium freudenreichii subsp. shermanii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Propionibacterium freudenreichii subsp. shermanii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-aspartate | natural substrate | Klebsiella aerogenes | fumarate + NH3 | - |
? | |
| L-aspartate | natural substrate | Propionibacterium freudenreichii subsp. shermanii | fumarate + NH3 | - |
? | |
| additional information | key enzyme involved in the metabolism of aspartate | Propionibacterium freudenreichii subsp. shermanii | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Propionibacterium freudenreichii subsp. shermanii |
| 8.5 | - |
- |
Klebsiella aerogenes |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | 0.6 | fumarate | - |
Propionibacterium freudenreichii subsp. shermanii | |
| 1.1 | 0.6 | fumarate | pH 7.5 | Propionibacterium freudenreichii subsp. shermanii | |
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