Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli and purified | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00021 | - |
25-mer-deoxyoligonucleotide 3'-labeled uracil-containing DNA duplex | NEIL1 | Mus musculus | |
0.0022 | - |
25-mer-deoxyoligonucleotide 3'-labeled uracil-containing DNA duplex | NEIL2 | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no effect my the presence or the absence of Mg2+. The activity of NEIL1 and NEIL2 are similar in potassium phosphate and Tris-HCl buffer | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38200 | - |
NEIL2, protein part of the enzyme-DNA complex, when trapped by the addition of NaBH4 to the dRP lyase activity assay. NaBH4 reduces the Schiff base formed between the catalytic nucleophile of dRP lyase and C1' of the dRP site | Mus musculus |
43500 | - |
NEIL1, protein part of the enzyme-DNA complex, when trapped by the addition of NaBH4 to the dRP lyase activity assay. NaBH4 reduces the Schiff base formed between the catalytic nucleophile of dRP lyase and C1' of the dRP site | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Mus musculus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
catalysis of the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site in DNA where a DNA-(apurinic or apyrimidinic site) lyase has already cleaved the C-O-P bond 3' to the apurinic or apyrimidinic site | NEIL1 and NEIL2 are capable of removing 2-deoxyribose 5-phosphate from DNA with the effiency comparable to that of Polbeta, and they can substitute for Polbeta dRPase activity in a reconstituted base excision repair (BER) system | Mus musculus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
NEIL1 and NEIL2 show similar efficiency, when A, C, or T are replaced opposite the lesion, and the excision opposite G was 1.5-2fold lower | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
25-mer-deoxyoligonucleotide 3'-labeled uracil-containing DNA duplex | [32P]-labeled substarte, treated with uracil-DNA glycosylase and apurinic/apyrimidinic endonuclease | Mus musculus | ? + 2-deoxy-D-ribose 5-phosphate | the products are stabilized by NaBH4 reduction of the Schiff base, formed between the catalytic nucleophile of the dRP lyase and C1' of rhe 2-deoxyribose 5-phosphate site, verifying the dRP removal by a beta-elimination | ? |
Synonyms | Comment | Organism |
---|---|---|
deoxyribo-5'-phosphate lyase | - |
Mus musculus |
dRP lyase | - |
Mus musculus |
dRPase | - |
Mus musculus |
mammalian endonuclease VIII-like protein | - |
Mus musculus |
NEIL1 | - |
Mus musculus |
NEIL2 | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Mus musculus |