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Literature summary for 4.2.99.22 extracted from
- Molecular diversity of tuliposide A-converting enzyme in the tulip (2013), Biosci. Biotechnol. Biochem., 77, 1042-1048.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| TCEA-b genes b1-b7 are higly homologous to each other, with six to seven single-nucleotide polymorphisms | Tulipa gesneriana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 12 | - |
6-tuliposide A | isoform TCEA-b1, pH not specified in the publication, temperature not specified in the publication | Tulipa gesneriana |  |
| 96 | - |
6-tuliposide B | isoform TCEA-b1, pH not specified in the publication, temperature not specified in the publication | Tulipa gesneriana | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plastid | - |
Tulipa gesneriana | 9536 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
2 * 34000, SDS-PAGE | Tulipa gesneriana |
| 87000 | - |
gel filtration | Tulipa gesneriana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tulipa gesneriana | R4X244 | isoform TCEA-b1 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bulb | expression of gene TCEA-b | Tulipa gesneriana | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1210 | - |
isoform TCEA-b1, pH not specified in the publication, temperature not specified in the publication | Tulipa gesneriana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-tuliposide A | i.e. 6-O-(4-hydroxy-2-methylenebutanoyl)-D-glucose | Tulipa gesneriana | tulipalin A + D-glucose | i.e. 2-methylenebutanolactone | ? | |
| 6-tuliposide B | - |
Tulipa gesneriana | tulipalin B + D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 34000, SDS-PAGE | Tulipa gesneriana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TCEA-b1 | - |
Tulipa gesneriana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 610 | - |
6-tuliposide B | isoform TCEA-b1, pH not specified in the publication, temperature not specified in the publication | Tulipa gesneriana | |
| 1400 | - |
6-tuliposide A | isoform TCEA-b1, pH not specified in the publication, temperature not specified in the publication | Tulipa gesneriana | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.4 | - |
6-tuliposide B | isoform TCEA-b1, pH not specified in the publication, temperature not specified in the publication | Tulipa gesneriana | |
| 120 | - |
6-tuliposide A | isoform TCEA-b1, pH not specified in the publication, temperature not specified in the publication | Tulipa gesneriana | |
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Release 2023.1 (January 2023)
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