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Literature summary for 4.2.99.21 extracted from

  • Gaille, C.; Kast, P.; Haas, D.
    Salicylate biosynthesis in Pseudomonas aeruginosa. Purification and characterization of PchB, a novel bifunctional enzyme displaying isochorismate pyruvate-lyase and chorismate mutase activities (2002), J. Biol. Chem., 277, 21768-21775.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
I88T no isochorismate pyruvate lyase activity, retains chorismate mutase activity Pseudomonas aeruginosa
additional information a CM-deficient Escherichia coli mutant, which is auxotrophic for phenylalanine and tyrosine, is functionally complemented by the cloned pchB gene for growth in minimal medium Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibitory at 1 mM: EDTA, EGTA, or o-phenanthroline. No substrate inhibition up to 1.2 mM Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0125
-
isochorismate pH 7.0, 37°C Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no metal cofactor requirement Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
11500
-
2 * 14000, SDS-PAGE, 2 * 11500, calculated Pseudomonas aeruginosa
14000
-
2 * 14000, SDS-PAGE, 2 * 11500, calculated Pseudomonas aeruginosa
30000
-
PAGE Pseudomonas aeruginosa
31000 34000 gel filtration Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.89
-
pH 7.0, 37°C Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isochorismate
-
Pseudomonas aeruginosa salicylate + pyruvate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 14000, SDS-PAGE, 2 * 11500, calculated Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.76
-
isochorismate pH 7.0, 37°C Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
additional information no cofactor required Pseudomonas aeruginosa