Protein Variants | Comment | Organism |
---|---|---|
I88T | no isochorismate pyruvate lyase activity, retains chorismate mutase activity | Pseudomonas aeruginosa |
additional information | a CM-deficient Escherichia coli mutant, which is auxotrophic for phenylalanine and tyrosine, is functionally complemented by the cloned pchB gene for growth in minimal medium | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibitory at 1 mM: EDTA, EGTA, or o-phenanthroline. No substrate inhibition up to 1.2 mM | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0125 | - |
isochorismate | pH 7.0, 37°C | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no metal cofactor requirement | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
11500 | - |
2 * 14000, SDS-PAGE, 2 * 11500, calculated | Pseudomonas aeruginosa |
14000 | - |
2 * 14000, SDS-PAGE, 2 * 11500, calculated | Pseudomonas aeruginosa |
30000 | - |
PAGE | Pseudomonas aeruginosa |
31000 | 34000 | gel filtration | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12.89 | - |
pH 7.0, 37°C | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isochorismate | - |
Pseudomonas aeruginosa | salicylate + pyruvate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 14000, SDS-PAGE, 2 * 11500, calculated | Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.76 | - |
isochorismate | pH 7.0, 37°C | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
- |
Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactor required | Pseudomonas aeruginosa |