Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.99.18 extracted from

  • Mueller, T.A.; Tobar, M.A.; Perian, M.N.; Hausinger, R.P.
    Biochemical characterization of AP lyase and m(6)A demethylase activities of human AlkB homologue 1 (ALKBH1) (2017), Biochemistry, 56, 1899-1910 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
analysis development of assay method using a methylation-sensitive restriction endonuclease Homo sapiens

Protein Variants

Protein Variants Comment Organism
C118A m6A demethylase activity similar to wild-type, about 25% decrease in apurinic/apyrimidinic lyase activity Homo sapiens
C118A/C129A about 50% decrease in m6A demethylase activity, about 20% decrease in apurinic/apyrimidinic lyase activity Homo sapiens
C129A about 90% decrease in m6A demethylase activity, about 30% decrease in apurinic/apyrimidinic lyase activity Homo sapiens
D233A no residual m6A demethylase activity, about 30% decrease in apurinic/apyrimidinic lyase activity Homo sapiens
H113A/C118A/C129A/H134A no residual m6A demethylase activity, about 55% decrease in apurinic/apyrimidinic lyase activity Homo sapiens
H231A no residual m6A demethylase activity, about 20% decrease in apurinic/apyrimidinic lyase activity Homo sapiens
H287A no residual m6A demethylase activity, about 25% decrease in apurinic/apyrimidinic lyase activity Homo sapiens
K133A about 50% decrease in m6A demethylase activity, about 45% decrease in apurinic/apyrimidinic lyase activity Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q13686 bifunctional apurinic/apyrimidinic sites lyase and m6A demethylase, cf. EC 1.14.11.51
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-fluorescein-labeled '-AGTAGACAAG(dU)TACCATGCCTGCACGAAGTT-3'
-
Homo sapiens ?
-
?
3'-fluorescein-labeled 5'-AACTTCGTGCAGGCATGGTAG(dU)TTGTCTACT-3'
-
Homo sapiens ?
-
?
3'-fluorescein-labeled 5'-AGTAGACAAGCTACCATGCCTGCACGAAGTT-3'
-
Homo sapiens ?
-
?
5'-AACTTCGTGCAGGCATGGG(m6A)TCTTGTCTACT-3'
-
Homo sapiens ?
-
?
5'-AGTAGACAAGATCCCATGCCTGCACGAAGTT-3'
-
Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
ALKBH1
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0005
-
3'-fluorescein-labeled 5'-AACTTCGTGCAGGCATGGTAG(dU)TTGTCTACT-3' pH 8, 37°C Homo sapiens

General Information

General Information Comment Organism
metabolism a nucleophilic residue of ALKBH1 reacts with the electrophilic C3' of the alpha,beta-unsaturated aldehyde of the 5'-product. The addition of a competing nucleophile, specifically 2-mercaptoethanol, reduces the extent of adduct formation Homo sapiens
physiological function the 6-methyl adenine demethylase activity is very low. The demethylase activity is less than half that of the apurinic/apyrimidinic lyase activity when ALKBH1 samples are assayed using identical buffer conditions. The two enzymatic activities are located in distinct but partially overlapping active sites for the two reactions Homo sapiens