Application | Comment | Organism |
---|---|---|
analysis | development of assay method using a methylation-sensitive restriction endonuclease | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C118A | m6A demethylase activity similar to wild-type, about 25% decrease in apurinic/apyrimidinic lyase activity | Homo sapiens |
C118A/C129A | about 50% decrease in m6A demethylase activity, about 20% decrease in apurinic/apyrimidinic lyase activity | Homo sapiens |
C129A | about 90% decrease in m6A demethylase activity, about 30% decrease in apurinic/apyrimidinic lyase activity | Homo sapiens |
D233A | no residual m6A demethylase activity, about 30% decrease in apurinic/apyrimidinic lyase activity | Homo sapiens |
H113A/C118A/C129A/H134A | no residual m6A demethylase activity, about 55% decrease in apurinic/apyrimidinic lyase activity | Homo sapiens |
H231A | no residual m6A demethylase activity, about 20% decrease in apurinic/apyrimidinic lyase activity | Homo sapiens |
H287A | no residual m6A demethylase activity, about 25% decrease in apurinic/apyrimidinic lyase activity | Homo sapiens |
K133A | about 50% decrease in m6A demethylase activity, about 45% decrease in apurinic/apyrimidinic lyase activity | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q13686 | bifunctional apurinic/apyrimidinic sites lyase and m6A demethylase, cf. EC 1.14.11.51 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-fluorescein-labeled '-AGTAGACAAG(dU)TACCATGCCTGCACGAAGTT-3' | - |
Homo sapiens | ? | - |
? | |
3'-fluorescein-labeled 5'-AACTTCGTGCAGGCATGGTAG(dU)TTGTCTACT-3' | - |
Homo sapiens | ? | - |
? | |
3'-fluorescein-labeled 5'-AGTAGACAAGCTACCATGCCTGCACGAAGTT-3' | - |
Homo sapiens | ? | - |
? | |
5'-AACTTCGTGCAGGCATGGG(m6A)TCTTGTCTACT-3' | - |
Homo sapiens | ? | - |
? | |
5'-AGTAGACAAGATCCCATGCCTGCACGAAGTT-3' | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ALKBH1 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
3'-fluorescein-labeled 5'-AACTTCGTGCAGGCATGGTAG(dU)TTGTCTACT-3' | pH 8, 37°C | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | a nucleophilic residue of ALKBH1 reacts with the electrophilic C3' of the alpha,beta-unsaturated aldehyde of the 5'-product. The addition of a competing nucleophile, specifically 2-mercaptoethanol, reduces the extent of adduct formation | Homo sapiens |
physiological function | the 6-methyl adenine demethylase activity is very low. The demethylase activity is less than half that of the apurinic/apyrimidinic lyase activity when ALKBH1 samples are assayed using identical buffer conditions. The two enzymatic activities are located in distinct but partially overlapping active sites for the two reactions | Homo sapiens |