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Literature summary for 4.2.99.18 extracted from
- Crystal structure of the crenarchaeal ExoIII AP endonuclease SisExoIII reveals a conserved disulfide bond endowing the protein with thermostability (2017), Biochem. Biophys. Res. Commun., 490, 774-779 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of enzyme mutant Y105A | Sulfolobus islandicus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| purified enzyme, sitting drop vapor diffusion method, a single crystal is obtained from 0.1 M bicine, pH 9.0, with 20% PEG 6000, at 16°C, X-ray diffraction structure determination and analysis, molecular replacement using the structure of Bacillus subtilis AP endonuclease ExoA (PDB ID 5CFE) as template | Sulfolobus islandicus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E233S | site-directed mutagenesis | Sulfolobus islandicus |
| Y105A | site-directed mutagenesis | Sulfolobus islandicus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | required | Sulfolobus islandicus |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfolobus islandicus | F0NEW7 | - |
- |
| Sulfolobus islandicus Rey15A | F0NEW7 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | tetrahydrofuran-containing DNA substrates are used | Sulfolobus islandicus | ? | - |
? | |
| additional information | tetrahydrofuran-containing DNA substrates are used | Sulfolobus islandicus Rey15A | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | SisExoIII comprises a two-layer core formed by 10 beta-sheets and a shell formed by 9 surrounding alpha-helices. A disulfide bond connecting beta8 and beta9 is formed by Cys142 and Cys215. Several key residues within the catalytic center including conserved Glu35 and Asn9 show different conformation compared with known ExoIII proteins and form various intra-molecular salt bridges. Three major DNA binding loops are predicted based on the distribution of electrostatic surface potential, positively charged loop 1 (Ile11-Lys16), loop 2 (Arg59-Tyr62), and loop 3 (Trp196-Leu208) | Sulfolobus islandicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AP endonuclease | - |
Sulfolobus islandicus |
| ExoIII | - |
Sulfolobus islandicus |
| SisExoIII | - |
Sulfolobus islandicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
assay at | Sulfolobus islandicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at | Sulfolobus islandicus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | disruption of the disulfide bond connecting beta8 and beta9 sheets only has a slight effect on the AP endonuclease activity | Sulfolobus islandicus |
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Release 2023.1 (January 2023)
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