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Literature summary for 4.2.99.18 extracted from

  • Onyango, D.O.; Naguleswaran, A.; Delaplane, S.; Reed, A.; Kelley, M.R.; Georgiadis, M.M.; Sullivan, W.J.
    Base excision repair apurinic/apyrimidinic endonucleases in apicomplexan parasite Toxoplasma gondii (2011), DNA Repair, 10, 466-475.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.99.18

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Toxoplasma gondii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0000262
-
 26-bp-oligonucleotide pH 7.5, 37°C Toxoplasma gondii

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Toxoplasma gondii 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ APN is active in presence and in absence of Mg2+ Toxoplasma gondii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
 x * 51000, SDS-PAGE Toxoplasma gondii

Organism

Organism UniProt Comment Textmining
Toxoplasma gondii F6KAZ5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
26-bp-oligonucleotide oligonucleotide containing a 5'-hexachloro-fluorescein phosphoramidite-labeled tetrahydrofuranyl residue in the middle Toxoplasma gondii 5'-hexachloro-fluorescein phosphoramidite-labeled 13-mer fragment + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 51000, SDS-PAGE Toxoplasma gondii

Synonyms

Synonyms Comment Organism
APN
-
 Toxoplasma gondii
APN1
-
 Toxoplasma gondii

General Information

General Information Comment Organism
physiological function over-expression of APN in Toxoplasma gondii confers protection from DNA damage, and viable knockouts of APN are not obtainable. An inducible APN knockdown mutant demonstrates that APN is critical for Toxoplasma to recover from DNA damage Toxoplasma gondii