Application | Comment | Organism |
---|---|---|
analysis | chemical footprinting-mass spectrometric assay using N-ethylmaleimide, an irreversible Cys modifier, to characterize the interaction of the redox inhibitor, E3330, with APE1. When APE1 is incubated with E3330, two N-ethylmaleimide-modified products are observed, one with two and a second with seven added N-ethylmaleimide molecules | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
E3330 | forms a reversible adduct with DELTA40APE1, an N-terminal truncation of APE1 including residues 40-318. E3330 also increases the extent of disulfide bond formation involving redox critical Cys residues in APE1 | Homo sapiens | |
N-ethylmaleimide | treatment of fully denatured full-length APE1 and DELTA40APE1, an N-terminal truncation of APE1 including residues 40-318, results in modification of 7 and 2 resiudes, respectively | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | APE1 adopts a partially unfolded state, which is proposed to be the redox active form of the enzyme | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
APE1 | - |
Homo sapiens |