Cloned (Comment) | Organism |
---|---|
UL30 is expressed in Spodoptera frugiperda cells | Human alphaherpesvirus 1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km for AP cleavage is similar to that for 5'-deoxyribose-5-phosphate removal, it explains the relative difference in apurinic/apyrimidinic cleavage observed with the plasmid and apurinic/apyrimidinic duplex oligonucleotide with the former containing a 100fold higher concentration of apurinic/apyrimidinic sites (100 uracil residues per plasmid molecule) than the oligonucleotides-based substrate. | Human alphaherpesvirus 1 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | cleavage of abasic DNA by UL30 occurs in the presence of EDTA and is independent of Mg2+, UL30 is 10fold less active | Human alphaherpesvirus 1 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
AP DNA | Human alphaherpesvirus 1 | AP sites | fragments of DNA | - |
? | |
additional information | Human alphaherpesvirus 1 | catalytic subunit of the HSV-1 DNA polymerase (Pol) (UL30) exhibits apurinic/apyrimidinic (AP) and 5'-deoxyribose phosphate lyase activities which are integral to base excision repair and lead to DNA cleavage on the 3'-side of abasic sites and 5'-deoxyribose-5-phosphate residues that remain after cleavage by 5'-AP endonuclease. DNA lyase activity residues in the Pol domain of UL30. | ? | - |
? | |
additional information | Human alphaherpesvirus 1 | enzyme has implications on the role of BER in viral genome maintenance during lytic replication and reactivation from latency | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Human alphaherpesvirus 1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Human alphaherpesvirus 1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
AP DNA | AP sites | Human alphaherpesvirus 1 | fragments of DNA | - |
? | |
linear 31-mer duplex oligonucleotide (3'-32P-labeled top strand contains an abasic site at position 17) | cleavage 3' to the apurinic/apyrimidinic site | Human alphaherpesvirus 1 | 3'-32P-labeled 14-mer oligonucleotide | - |
? | |
additional information | catalytic subunit of the HSV-1 DNA polymerase (Pol) (UL30) exhibits apurinic/apyrimidinic (AP) and 5'-deoxyribose phosphate lyase activities which are integral to base excision repair and lead to DNA cleavage on the 3'-side of abasic sites and 5'-deoxyribose-5-phosphate residues that remain after cleavage by 5'-AP endonuclease. DNA lyase activity residues in the Pol domain of UL30. | Human alphaherpesvirus 1 | ? | - |
? | |
additional information | enzyme has implications on the role of BER in viral genome maintenance during lytic replication and reactivation from latency | Human alphaherpesvirus 1 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
apurinic/apyrimidinic lyase | catalytic subunit of the HSV-1 DNA polymerase (Pol) (UL30) (EC 2.7.7.7) exhibits apurinic/apyrimidinic and 5'-deoxyribose phosphate lyase activities | Human alphaherpesvirus 1 |
DNA lyase | - |
Human alphaherpesvirus 1 |
UL30 | - |
Human alphaherpesvirus 1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Human alphaherpesvirus 1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Human alphaherpesvirus 1 |