Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.99.18 extracted from

  • Kim, M.H.; Kim, H.B.; Acharya, S.; Sohn, H.M.; Jun, J.Y.; Chang, I.Y.; You, H.J.
    Ape1/Ref-1 induces GDNF responsiveness by upregulating GFRalpha1 expression (2009), Mol. Cell. Biol., 29, 2264-2277.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.99.18

Cloned(Commentary)

Cloned (Comment) Organism
Human Ape1/Ref-1 cDNA is amplified by RT-PCR using Ape1/Ref-1-specific primers and from human GM00637 fibroblasts. AP endonuclease domain deletion mutant deltaAPD is amplified by RT-PCR using specific primers from full length human Ape1/Ref-1 cDNA. Redox domain deletion mutant deltaRD is amplified by RT-PCR using specific primers from full length human Ape1/Ref-1 cDNA. Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens enzyme cleaves the AP sites in DNA and allows them to be repaired by other enzymes involved in base excision repair ?
-
 ?
additional information Homo sapiens enzyme stimulates the DNA binding activity of the AP-1 family of transcription factors via a redox-dependent mechanism ?
-
 ?
additional information Homo sapiens the DNA-binding ability of NF-kappaB in the Ape1/Ref-1 expressing cells is significantly increased ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
GM00637 cell fibroblasts Homo sapiens
-
pancreatic cancer cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 Ape1/Ref-1 increases the expression of glial cell-derived neurotropic factor receptor alpha1 (GFRalpha1), a key receptor for glial cell-derived neurotropic factor (GDNF). Expression of Ape1/Ref- 1 leads to an increase in the GDNF responsiveness in human fibroblast. Ape1/Ref-1 induced GFRalpha1 transcription through enhances binding of NF-kappaB complexes to the GFRalpha1 promoter. Homo sapiens
additional information
-
 GFRalpha1 levels correlates proportionally with Ape1/Ref-1 in cancer cells Homo sapiens
additional information
-
 in neuronal cells, the Ape1/Ref-1-mediated increase in GDNF responsiveness not only stimulated neurite outgrowth but also protected the cells from beta-amyloid peptide and oxidative stress Homo sapiens
additional information
-
 the knockdown of endogenous Ape1/Ref-1 in pancreatic cancer cells markedly suppresses GFRalpha1 expression and invasion in response to GNDF, while overexpression of GFRalpha1 restores invasion Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme cleaves the AP sites in DNA and allows them to be repaired by other enzymes involved in base excision repair Homo sapiens ?
-
 ?
additional information enzyme stimulates the DNA binding activity of the AP-1 family of transcription factors via a redox-dependent mechanism Homo sapiens ?
-
 ?
additional information the DNA-binding ability of NF-kappaB in the Ape1/Ref-1 expressing cells is significantly increased Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
APE1/Ref-1
-
 Homo sapiens
apurinic/apyrimidinic endonuclease 1
-
 Homo sapiens
redox factor-1:Ref-1
-
 Homo sapiens