Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | in presence of 1 mM Mg2+, Ape1 abasic endonuclease activity is stimulated (1.75- and 1.25-fold at 0.5 and 1 mM ATP concentrations). At 10 mM MgCl2, the higher concentrations of ATP has stimulatory effects on AP site incision by Ape1 | Homo sapiens | |
DNA-dependent ATPase CSB | in presence of 1 mM Mg2+, enzyme stimulates AP site incision by Ape1 in the absence of ATP on both fully paired duplex DNA (42F-42Comp) and an 11-nt bubble duplex substrate containing a centrally located abasic site (42F-42bubbleComp). When 2.5 mM ATP is included in the reactions with 1 mM MgCl2, inhibition of Ape1 incision activity is detected | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | in presence of 1 mM Mg2+, Ape1 incision activity is inhibited at higher ATP concentrations (2-5 mM). Depending on the relative concentration of Mg2+, ATP can have both inhibitory and stimulatory consequences on Ape1 incision capacity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Steady-state kinetic analysis of Ape1 AP endonuclease activity at 10 mM Mg2+ with and without 5 mM ATP indicates a less than 2fold difference in KM-value but an 19fold enhancement in kcat in the presence of ATP, suggesting an enhancement of the catalytic reaction specifically | Homo sapiens | |
0.0000057 | - |
AP-DNA-RNA | 1 mM Mg2+ | Homo sapiens | |
0.0000238 | - |
AP-DNA-DNA | 1 mM Mg2+ | Homo sapiens | |
0.0001792 | - |
34FDNA | - |
Homo sapiens | |
0.001561 | - |
34FRNA | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | both MgCl2 and KCl strongly influence the efficiency of Ape1 as an ssDNA or dsDNA AP endonuclease | Homo sapiens | |
Mg2+ | In presence of Mg2+, ATP has complex effects on Ape1 cleavage activity. Endo- and exonuclease activity of Ape1 is found to be influenced by the MgCl2 concentration, with optimal exonuclease activity at low MgCl2 concentration (0.1-2 mM) and optimal endonuclease activity at high MgCl2 concentration (10-15 mM). | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | Ape1 has the ability to incise at AP sites in DNA conformations formed during DNA replication, transcription, and class switch recombination, and that Ape1 can endonucleolytically destroy damaged RNA | ? | - |
? | |
additional information | Homo sapiens | Ape1 is a multifunctional enzyme of 318 amino acids with redox-dependent regulation of transcription factors, 3' to 5' exonuclease, 3' phosphodiesterase, RNaseH and class II type AP endonuclease activities | ? | - |
? | |
additional information | Homo sapiens | human Ape1 is a multifunctional protein with a major role in initiating repair of apurinic/apyrimidinic (AP) sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
Ape1 has only slightly more robust activity on the partial DNA-DNA duplex than on the comparable DNA/RNA duplex. | Homo sapiens |
additional information | - |
creation of three-strand substrates provided a 2- to 2.5fold increase in the specific activity of Ape1 over the partial duplex with the abasic lesion positioned at the ssDNA-dsDNA junction (54Fendbubble18-18DNA) | Homo sapiens |
additional information | - |
substrates with either a purine (G) or a pyrimidine (C) positioned opposite the AP site and two purines flanking the abasic site shows that Ape1 incision activity under optimal reaction conditions is two to threefold higher with the GFT-CCA, CFA-GCT, and CFT-GGA arrangements relative to GFA-CCT | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
34FDNA | - |
Homo sapiens | ? | - |
? | |
34FRNA | - |
Homo sapiens | ? | - |
? | |
AP-DNA-DNA | synthetic DNA-DNA hybrid | Homo sapiens | ? | - |
? | |
AP-DNA-RNA | synthetic DNA-RNA hybrids that simulate a transcription intermediate | Homo sapiens | ? | - |
? | |
additional information | Ape1 has the ability to incise at AP sites in DNA conformations formed during DNA replication, transcription, and class switch recombination, and that Ape1 can endonucleolytically destroy damaged RNA | Homo sapiens | ? | - |
? | |
additional information | Ape1 is a multifunctional enzyme of 318 amino acids with redox-dependent regulation of transcription factors, 3' to 5' exonuclease, 3' phosphodiesterase, RNaseH and class II type AP endonuclease activities | Homo sapiens | ? | - |
? | |
additional information | human Ape1 is a multifunctional protein with a major role in initiating repair of apurinic/apyrimidinic (AP) sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage | Homo sapiens | ? | - |
? | |
additional information | Used oligonucleotides are 54mers annealed to a complementary 18mer DNA to form the partial duplex substrates (54F-endbubble or 54F-centerbubble with 18DNA) and two 54mers annealed to create an 18-nt bubble structure (54Fendbubble or 54Fcenterbubble with 54bubble18comp). The abasic site is either centrally located (center bubble DNAs) or located at the ssDNA-dsDNA junction (end bubble DNAs). Ape1 most efficiently incised at an abasic site located centrally in the 18-nt bubble structure (54Fcenterbubble18-54bubblecomp), followed by the centrally located abasic site in the partial duplex DNA (54Fcenterbubble18-18DNA), the abasic site at an ssDNA/dsDNA junction in the bubble conformation (54Fendbubble18-54bubblecomp), and the abasic site at an ssDNA/dsDNA junction in partial duplex DNA (54Fendbubble18-18DNA) | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
APE1 | - |
Homo sapiens |
apurinic/Apyrimidinic (AP) endonuclease 1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
54 | - |
AP-DNA-RNA | - |
Homo sapiens | |
174 | - |
AP-DNA-DNA | - |
Homo sapiens | |
438 | - |
34FRNA | - |
Homo sapiens | |
26500 | - |
34FDNA | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Homo sapiens |