Literature summary for 4.2.99.18 extracted from

Watanabe, T.; Blaisdell, J.O.; Wallace, S.S.; Bond, J.P.
Engineering functional changes in Escherichia coli endonuclease III based on phylogenetic and structural analyses (2005), J. Biol. Chem., 280, 34378-34384.

Search for more literature for 4.2.99.18

Protein Variants

Protein Variants	Comment	Organism
D44V	mutant retains glycosylase activity against oxidized pyrimidines, but the apparent rate constant for the lyase activity is significantly lower than the wild-type value	Escherichia coli
R184A	mutant enzyme maintains lyase activity but exhibits glycosylase specificity different from wild-type enzyme	Escherichia coli
S39L	mutant does not have significant glycosylase activity for oxidized pyrimidines, alhough it maintains AP lysae activity	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0000036	-	35 base pair oligonucleotide containing 5,6-dihydrouracil opposite G	pH 7.6, 37°C, wild-type enzyme	Escherichia coli
0.0000059	-	35 base pair oligonucleotide containing 5,6-dihydroxy-5,6-dihydrothymine opposite G	pH 7.6, 37°C, wild-type enzyme	Escherichia coli
0.0000091	-	35 base pair oligonucleotide containing 5,6-dihydrouracil opposite A	pH 7.6, 37°C, wild-type enzyme	Escherichia coli
0.0000097	-	35 base pair oligonucleotide containing 5,6-dihydroxy-5,6-dihydrothymine opposite A	pH 7.6, 37°C, wild-type enzyme	Escherichia coli
0.000133	-	35 base pair oligonucleotide containing 5,6-dihydrouracil opposite G	pH 7.6, 37°C, mutant enzyme R184A	Escherichia coli
0.00192	-	35 base pair oligonucleotide containing 5,6-dihydroxy-5,6-dihydrothymine opposite G	pH 7.6, 37°C, mutant enzyme R184A	Escherichia coli
0.004	-	35 base pair oligonucleotide containing 5,6-dihydroxy-5,6-dihydrothymine opposite A	pH 7.6, 37°C, mutant enzyme R184A	Escherichia coli
0.14	-	35 base pair oligonucleotide containing 5,6-dihydrouracil opposite A	pH 7.6, 37°C, mutant enzyme R184A	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
35 base pair oligonucleotide containing 5,6-dihydrouracil opposite A	-	Escherichia coli	?	-	?
35 base pair oligonucleotide containing 5,6-dihydrouracil opposite G	-	Escherichia coli	?	-	?
35 base pair oligonucleotide containing 5,6-dihydroxy-5,6-dihydrothymine opposite A	-	Escherichia coli	?	-	?
35 base pair oligonucleotide containing 5,6-dihydroxy-5,6-dihydrothymine opposite G	-	Escherichia coli	?	-	?
DNA containing apurinic/apyrimidinic sites	endonuclease III plays an important cellular role by removing premutagenic pyrimidine damages produced by reactive oxygen species. EcoNth is a bifunctional enzyme that has DNA glycosylase and apurinic/apyrimidinic lyase activity	Escherichia coli	fragments of DNA	-	?

Synonyms

Synonyms	Comment	Organism
EcoNth	a bifunctional enzyme that has DNA glycosylase and apurinic/apyrimidinic lyase activity	Escherichia coli
endonuclease III	-	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.03	-	35 base pair oligonucleotide containing 5,6-dihydrouracil opposite A	pH 7.6, 37°C, wild-type enzyme	Escherichia coli
0.043	-	35 base pair oligonucleotide containing 5,6-dihydrouracil opposite G	pH 7.6, 37°C, wild-type enzyme	Escherichia coli
0.075	-	35 base pair oligonucleotide containing 5,6-dihydroxy-5,6-dihydrothymine opposite G	pH 7.6, 37°C, wild-type enzyme	Escherichia coli
0.098	-	35 base pair oligonucleotide containing 5,6-dihydroxy-5,6-dihydrothymine opposite A	pH 7.6, 37°C, wild-type enzyme	Escherichia coli
0.098	-	35 base pair oligonucleotide containing 5,6-dihydroxy-5,6-dihydrothymine opposite A	pH 7.6, 37°C, mutant enzyme R184A	Escherichia coli
0.17	-	35 base pair oligonucleotide containing 5,6-dihydrouracil opposite G	pH 7.6, 37°C, mutant enzyme R184A	Escherichia coli
1.37	-	35 base pair oligonucleotide containing 5,6-dihydrouracil opposite A	pH 7.6, 37°C, mutant enzyme R184A	Escherichia coli
1.6	-	35 base pair oligonucleotide containing 5,6-dihydroxy-5,6-dihydrothymine opposite G	pH 7.6, 37°C, mutant enzyme R184A	Escherichia coli

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Release 2023.1 (January 2023)