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Literature summary for 4.2.99.18 extracted from
- Human AP endonuclease (APE1) demonstrates endonucleolytic activity against AP sites in single-stranded DNA (2004), DNA Repair, 3, 527-533.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D210N | mutant enzyme is catalytically inactive against abasic sites in double-stranded DNA and single-stranded DNA | Homo sapiens |
| DELTA1-29 | mutant enzyme retains activity against abasic sites in single-stranded DNA | Homo sapiens |
| DELTA1-36 | mutant enzyme retains activity against abasic sites in single-stranded DNA | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | in contrast to activity against abasic sites in souble-stranded DNA the enzyme does not display product inhibition when acting on absic sites in single-stranded DNA | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000024 | - |
double-stranded DNA with abasic sites | - |
Homo sapiens | |
| 0.000428 | - |
single-stranded DNA with abasic sites | - |
Homo sapiens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activity against abasic sites in single-stranded DNA | Homo sapiens |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded DNA with abasic sites | - |
Homo sapiens | ? | - |
? | |
| additional information | the enzyme remains bound to its incision product when the 5'-incision us in double-stranded DNA. The enzyme dissociates from its single-stranded 5'-incised product | Homo sapiens | ? | - |
? | |
| single-stranded DNA with abasic sites | catalytic efficiency is 20fold less than the activity against double-stranded DNA with abasic sites | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AP endonuclease | - |
Homo sapiens |
| APE1 | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.1 | - |
double-stranded DNA with abasic sites | - |
Homo sapiens | |
| 4.2 | - |
single-stranded DNA with abasic sites | - |
Homo sapiens |
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