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Literature summary for 4.2.99.18 extracted from

  • Mguyen, L.H.; Barsky, D.; Erzberger, J.P.; Wilson, D.M., 3rd
    Mapping the protein-DNA interface and the metal-binding site of the major human apurinic/apyrimidinic endonuclease (2000), J. Mol. Biol., 298, 447-459.
    View publication on PubMed
Search for more literature for 4.2.99.18

Protein Variants

Protein Variants Comment Organism
D210N catalytically inactive Homo sapiens
D70R binding affinity nearly identical with wild-type enzyme, reduced specific endonuclease activity, at Mg2+ concentrations below 1 mM the activity of the mutant sharply decreases Homo sapiens
E96Q binding affinity nearly identical with wild-type enzyme, reduced specific endonuclease activity Homo sapiens
N68A binding affinity nearly identical with wild-type enzyme, reduced specific endonuclease activity, at Mg2+ concentrations below 1 mM the activity of the mutant sharply decreases Homo sapiens
R156Q 100fold reduced DNA binding capacity Homo sapiens
Y128A 100fold reduced DNA binding capacity Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ Fe2+ is able to support the incision activity of the enzyme at excess protein to DNA ratios of at least 6:1, Mg2+ and Fe2+ compete for the same metal-binding site Homo sapiens
Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA Homo sapiens
-
 fragments of DNA
-
 ?
additional information Homo sapiens corrects apurinic/apyrimidinic sites in the genome ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Reaction

Reaction Comment Organism Reaction ID
the C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate mechanism Homo sapiens
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA
-
 Homo sapiens fragments of DNA
-
 ?
additional information corrects apurinic/apyrimidinic sites in the genome Homo sapiens ?
-
 ?