Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
DELTAP211 | truncated hairpin, mutant protein is inactive | Homo sapiens |
InsG212 | extended hairpin, diminished recognition and binding to 5-hydroxycytosine-containing DNA | Homo sapiens |
K212S | mutant protein is inactive | Homo sapiens |
P211R | kinetic parameters similar to wild-type enzyme, decreased specificity of binding | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | 5-OH-C paired with adenine was poorly repaired with increasing Mg2+ concentrations, no incision of 5-OH-C opposite adenine above 15 mM Mg2+ | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000000048 | - |
DNA containing 5-OH-C/G | P211R mutant, pH 7.5, 37°C, presence of Mg2+ | Homo sapiens | |
0.00000005 | - |
DNA containing 5-OH-C/G | wild-type, pH 7.5, 37°C, presence of Mg2+ or EDTA | Homo sapiens | |
0.00000007 | - |
DNA containing 5-OH-C/A | wild-type, pH 7.5, 37°C, presence of EDTA | Homo sapiens | |
0.00000009 | - |
DNA containing 5-OH-C/G | P211R mutant, pH 7.5, 37°C, presence of EDTA | Homo sapiens | |
0.00000014 | - |
DNA containing 5-OH-C/A | P211R mutant, pH 7.5, 37°C, presence of Mg2+ | Homo sapiens | |
0.0000002 | - |
DNA containing 5-OH-C/A | P211R mutant, pH 7.5, 37°C, presence of EDTA | Homo sapiens | |
0.0000002 | - |
DNA containing 5-OH-C/A | wild-type, pH 7.5, 37°C, presence of Mg2+ | Homo sapiens | |
0.000001 | - |
DNA containing 5-OH-C/G | G212 mutant, pH 7.5, 37°C, presence of EDTA | Homo sapiens | |
0.0000012 | - |
DNA containing 5-OH-C/G | G212 mutant, pH 7.5, 37°C, presence of Mg2+ | Homo sapiens | |
0.0000017 | - |
DNA containing 5-OH-C/A | G212 mutant, pH 7.5, 37°C, presence of Mg2+ | Homo sapiens | |
0.0000035 | - |
DNA containing 5-OH-C/A | G212 mutant, pH 7.5, 37°C, presence of EDTA | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | repair of 5-OH-C opposite guanine is stimulated 2-fold with increasing concentrations, 5-OH-C paired with adenine is poorly repaired with increasing Mg2+ concentrations, no incision of 5-OH-C opposite adenine above 15 mM Mg2+ | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA | Homo sapiens | - |
fragments of DNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA | - |
Homo sapiens | fragments of DNA | - |
? | |
DNA | acts on both 5-hydroxycytosine and abasic sites, preferentially when these are situated opposite guanines | Homo sapiens | fragments of DNA | - |
? | |
DNA containing 5-OH-C/A | - |
Homo sapiens | ? | - |
? | |
DNA containing 5-OH-C/G | - |
Homo sapiens | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000035 | - |
DNA containing 5-OH-C/A | + G 212 mutant, pH 7.5, 37°C, presence of Mg2+ | Homo sapiens | |
0.0000667 | - |
DNA containing 5-OH-C/A | + G 212 mutant, pH 7.5, 37°C, presence of EDTA | Homo sapiens | |
0.0001 | - |
DNA containing 5-OH-C/A | P211R mutant, pH 7.5, 37°C, presence of Mg2+ | Homo sapiens | |
0.000233 | - |
DNA containing 5-OH-C/G | + G 212 mutant, pH 7.5, 37°C, presence of Mg2+ | Homo sapiens | |
0.000333 | - |
DNA containing 5-OH-C/A | wild-type, pH 7.5, 37°C, presence of Mg2+ | Homo sapiens | |
0.0005 | - |
DNA containing 5-OH-C/G | P211R mutant, pH 7.5, 37°C, presence of Mg2+ | Homo sapiens | |
0.0005 | - |
DNA containing 5-OH-C/A | P211R mutant, pH 7.5, 37°C, presence of EDTA | Homo sapiens | |
0.0005 | - |
DNA containing 5-OH-C/A | wild-type, pH 7.5, 37°C, presence of EDTA | Homo sapiens | |
0.000583 | - |
DNA containing 5-OH-C/G | P211R mutant, pH 7.5, 37°C, presence of EDTA | Homo sapiens | |
0.000667 | - |
DNA containing 5-OH-C/G | wild-type, pH 7.5, 37°C, presence of EDTA | Homo sapiens | |
0.000833 | - |
DNA containing 5-OH-C/G | wild-type, pH 7.5, 37°C, presence of Mg2+ | Homo sapiens | |
0.00117 | - |
DNA containing 5-OH-C/G | + G 212 mutant, pH 7.5, 37°C, presence of EDTA | Homo sapiens |