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Literature summary for 4.2.99.18 extracted from

  • Eide, L.; Luna, L.; Gustad, E.C.; Henderson, P.T.; Essigmann, J.M.; Demple, B.; Seeberg, E.
    Human endonuclease III acts preferentially on DNA damage opposite guanine residues in DNA (2001), Biochemistry, 40, 6653-6659.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Homo sapiens

Protein Variants

Protein Variants Comment Organism
DELTAP211 truncated hairpin, mutant protein is inactive Homo sapiens
InsG212 extended hairpin, diminished recognition and binding to 5-hydroxycytosine-containing DNA Homo sapiens
K212S mutant protein is inactive Homo sapiens
P211R kinetic parameters similar to wild-type enzyme, decreased specificity of binding Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information 5-OH-C paired with adenine was poorly repaired with increasing Mg2+ concentrations, no incision of 5-OH-C opposite adenine above 15 mM Mg2+ Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000000048
-
DNA containing 5-OH-C/G P211R mutant, pH 7.5, 37°C, presence of Mg2+ Homo sapiens
0.00000005
-
DNA containing 5-OH-C/G wild-type, pH 7.5, 37°C, presence of Mg2+ or EDTA Homo sapiens
0.00000007
-
DNA containing 5-OH-C/A wild-type, pH 7.5, 37°C, presence of EDTA Homo sapiens
0.00000009
-
DNA containing 5-OH-C/G P211R mutant, pH 7.5, 37°C, presence of EDTA Homo sapiens
0.00000014
-
DNA containing 5-OH-C/A P211R mutant, pH 7.5, 37°C, presence of Mg2+ Homo sapiens
0.0000002
-
DNA containing 5-OH-C/A P211R mutant, pH 7.5, 37°C, presence of EDTA Homo sapiens
0.0000002
-
DNA containing 5-OH-C/A wild-type, pH 7.5, 37°C, presence of Mg2+ Homo sapiens
0.000001
-
DNA containing 5-OH-C/G G212 mutant, pH 7.5, 37°C, presence of EDTA Homo sapiens
0.0000012
-
DNA containing 5-OH-C/G G212 mutant, pH 7.5, 37°C, presence of Mg2+ Homo sapiens
0.0000017
-
DNA containing 5-OH-C/A G212 mutant, pH 7.5, 37°C, presence of Mg2+ Homo sapiens
0.0000035
-
DNA containing 5-OH-C/A G212 mutant, pH 7.5, 37°C, presence of EDTA Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ repair of 5-OH-C opposite guanine is stimulated 2-fold with increasing concentrations, 5-OH-C paired with adenine is poorly repaired with increasing Mg2+ concentrations, no incision of 5-OH-C opposite adenine above 15 mM Mg2+ Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA Homo sapiens
-
fragments of DNA
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA
-
Homo sapiens fragments of DNA
-
?
DNA acts on both 5-hydroxycytosine and abasic sites, preferentially when these are situated opposite guanines Homo sapiens fragments of DNA
-
?
DNA containing 5-OH-C/A
-
Homo sapiens ?
-
?
DNA containing 5-OH-C/G
-
Homo sapiens ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000035
-
DNA containing 5-OH-C/A + G 212 mutant, pH 7.5, 37°C, presence of Mg2+ Homo sapiens
0.0000667
-
DNA containing 5-OH-C/A + G 212 mutant, pH 7.5, 37°C, presence of EDTA Homo sapiens
0.0001
-
DNA containing 5-OH-C/A P211R mutant, pH 7.5, 37°C, presence of Mg2+ Homo sapiens
0.000233
-
DNA containing 5-OH-C/G + G 212 mutant, pH 7.5, 37°C, presence of Mg2+ Homo sapiens
0.000333
-
DNA containing 5-OH-C/A wild-type, pH 7.5, 37°C, presence of Mg2+ Homo sapiens
0.0005
-
DNA containing 5-OH-C/G P211R mutant, pH 7.5, 37°C, presence of Mg2+ Homo sapiens
0.0005
-
DNA containing 5-OH-C/A P211R mutant, pH 7.5, 37°C, presence of EDTA Homo sapiens
0.0005
-
DNA containing 5-OH-C/A wild-type, pH 7.5, 37°C, presence of EDTA Homo sapiens
0.000583
-
DNA containing 5-OH-C/G P211R mutant, pH 7.5, 37°C, presence of EDTA Homo sapiens
0.000667
-
DNA containing 5-OH-C/G wild-type, pH 7.5, 37°C, presence of EDTA Homo sapiens
0.000833
-
DNA containing 5-OH-C/G wild-type, pH 7.5, 37°C, presence of Mg2+ Homo sapiens
0.00117
-
DNA containing 5-OH-C/G + G 212 mutant, pH 7.5, 37°C, presence of EDTA Homo sapiens