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Literature summary for 4.2.99.18 extracted from

  • Frost, B.F.; Small, G.D.
    Partial purification and characterization of the major endonuclease from Chlamydomonas reinhardtii (1984), Biochim. Biophys. Acta, 782, 170-176.
    View publication on PubMed
Search for more literature for 4.2.99.18

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol stimulates over 100% at 1 mM Chlamydomonas reinhardtii
Triton X-100 20-50% stimulation at 0.03% Chlamydomonas reinhardtii

Inhibitors

Inhibitors Comment Organism Structure
KCl 50 mM: 70% inhibition Chlamydomonas reinhardtii
NaCl 50 mM: 70% inhibition Chlamydomonas reinhardtii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Mg2+ or Mn2+ required, Mg2+ better than Mn2+ Chlamydomonas reinhardtii
Mn2+ Mn2+ or Mg2+ required Chlamydomonas reinhardtii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 gel filtration Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA Chlamydomonas reinhardtii
-
 fragments of DNA
-
 ?

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA
-
 Chlamydomonas reinhardtii fragments of DNA
-
 ?
DNA specific for DNA containing either apurinic or apyrimidinic sites Chlamydomonas reinhardtii fragments of DNA
-
 ?
additional information no activity with DNA that has been damaged by UV light, methyl methanesulfonate, osmium teroxide or sodium bisulfite Chlamydomonas reinhardtii ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 8.2
-
 Chlamydomonas reinhardtii