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Literature summary for 4.2.99.18 extracted from
- Purification and properties of a Bacillus subtilis endonuclease specific for apurinic sites in DNA (1978), J. Biol. Chem., 253, 8559-8563.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-chloromercuribenzoate | complete inhibition at 2 mM | Bacillus subtilis |  |
| EDTA | no inhibition | Bacillus subtilis | |
| NaCl | above 50 mM: slight stimulation, 500 mM: complete inhibition | Bacillus subtilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stimulates | Bacillus subtilis | |
| Mg2+ | stimulates | Bacillus subtilis | |
| Mn2+ | stimulates | Bacillus subtilis | |
| NaCl | 50 mM: slight stimulation, 500 mM: complete inhibition | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
gel filtration | Bacillus subtilis |
| 56000 | - |
1 * 56000, SDS-PAGE | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA | Bacillus subtilis | - |
fragments of DNA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA | - |
Bacillus subtilis | fragments of DNA | - |
? | |
| DNA | specific for apurinic sites | Bacillus subtilis | fragments of DNA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 56000, SDS-PAGE | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Bacillus subtilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | 8.6 | about 80% of activity maximum at pH 7.4 and pH 8.2, 62% of maximum activity at pH 8.6 | Bacillus subtilis |
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