Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16000 | - |
bacteriophage T4 infected, SDS-PAGE, T4 endonuclease V (EC 3.1.25.1) possesses both pyrimidine dimer DNA glycosylase activity and apurinic/apyrimidinic DNA endonuclease EC 4.2.99.18 activity in a single polypeptide chain | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA | Escherichia coli | - |
fragments of DNA | - |
? | |
DNA | Tequatrovirus T4 | - |
fragments of DNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
bacteriophage T4 infected, T4 endonuclease V (EC 3.1.25.1) possesses both pyrimidine dimer DNA glycosylase activity and apurinic/apyrimidinic DNA endonuclease (EC 4.2.99.18) activity in a single polypeptide chain | - |
Tequatrovirus T4 | - |
Escherichia coli infected with bacteriophage T4, T4 endonuclease V (EC 3.1.25.1) possesses both pyrimidine dimer DNA glycosylase activity and apurinic/apyrimidinic DNA endonuclease (E.C. 3.1.25.2) activity in a single polypeptide chain | - |
Purification (Comment) | Organism |
---|---|
- |
Tequatrovirus T4 |
infected with bacteriophage T4 | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
the C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate | T4 endonuclease V (EC 3.1.25.1) possesses both pyrimidine dimer DNA glycosylase activity and apurinic/apyrimidinic DNA endonuclease (EC 4.2.99.18) activity in a single polypeptide chain | Tequatrovirus T4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA | - |
Escherichia coli | fragments of DNA | - |
? | |
DNA | - |
Tequatrovirus T4 | fragments of DNA | - |
? | |
DNA | endonucleolytic cleavage near apurinic or apyrimidinic sites to products with 5'-phosphates, e.g. UV-irradiated poly(dA)*poly(dT) | Escherichia coli | fragments of DNA | - |
? | |
DNA | endonucleolytic cleavage near apurinic or apyrimidinic sites to products with 5'-phosphates, e.g. UV-irradiated poly(dA)*poly(dT) | Tequatrovirus T4 | fragments of DNA | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
5 min, 70% loss of activity | Escherichia coli |
42 | - |
5 min, 70% loss of activity | Tequatrovirus T4 |